Physical activity improves age-related decline in the osteogenic potential of rats' bone marrow-derived mesenchymal stem cells
Physical activity improves age-related decline in the osteogenic potential of rats' bone marrow-derived mesenchymal stem cells
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DOI：
10.1111/j.1748-1716.2011.02397.x
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发表时间：
2012-06-01
影响因子：
6.3
通讯作者：
Serakides, R.
中科院分区：
文献类型：
--
作者：
Hell, R. C. R.;Ocarino, N. M.;Serakides, R.
目的：探讨运动对成年大鼠骨髓间充质干细胞(BMMSCs)成骨分化的促进作用。方法：18只雌性Wistar大鼠随机分为3组：(1)青年供体BMMSCs，(2)久坐不动供体BMMSCs，(3)活动供体BMMSCs。结果：成年大鼠骨髓间充质干细胞的端粒酶逆转录酶、碱性磷酸酶、半胱氨酸天冬氨酸氨基转移酶3、骨钙素、骨涎蛋白和I型胶原的基因表达水平和矿化结节数均低于青年供者。而成年供者骨髓间充质干细胞中caspase 3的表达水平明显高于成人供者(P
Aim: To examine whether physical activity increases osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) from adult rats compared with young rats.Methods: Eighteen female Wistar rats were divided into three groups and the following cells isolated: (1) differentiated BMMSCs from young donors, (2) differentiated BMMSCs from sedentary adult donors and (3) differentiated BMMSCs from active adult donors. We analysed MTT conversion, percentage of cells per field, mineralized nodule number and gene expression for telomerase reverse transcriptase (TERT), alkaline phosphatase, caspase 3, osteocalcin, bone sialoprotein and collagen I.Results: Telomerase reverse transcriptase expression and the percentage of cells per field in BMMSCs cultures from adult rats were smaller than those observed in young donors. However, levels of caspase 3 expression were higher in BMMSCs from adult donors (P