Argonaute 1 regulates the fate of germline stem cells in Drosophila
Argonaute 1 regulates the fate of germline stem cells in Drosophila
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DOI：
10.1242/dev.009159
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发表时间：
2007-12-01
期刊：
影响因子：
4.6
通讯作者：
Chen, Dahua
中科院分区：
文献类型：
--
作者：
Yang, Lele;Chen, Dongsheng;Chen, Dahua
Argonaute家族蛋白在小RNA介导的基因调控中起着至关重要的作用。在果蝇中，先前的研究已经证明Piwi，Argonaute蛋白PIWI亚家族的一个成员，在调节生殖系干细胞（GSC）的命运中起着至关重要的作用。然而，其他Argonaute蛋白是否也发挥类似的作用仍然难以捉摸。在这里，我们表明，Argonaute 1（AGO 1）蛋白，Argonaute蛋白的另一个亚家族（AGO）的过度表达，导致GSC过度增殖，而Ago 1的损失导致GSC的损失。结合Ago 1的生殖系克隆分析，这些发现有力地支持了Ago 1在维持GSC中起着重要和内在作用的论点。与先前观察到的Piwi在维持GSC中的功能相反，我们发现AGO 1不是弹珠袋（BAM）沉默所需的，并且可能在GSC命运的调节中作用于BAM的下游或平行。鉴于AGO 1作为miRNA通路的关键组成部分，我们认为AGO 1依赖的miRNA通路可能在抑制GSC/成囊细胞分化中起着指导作用。
The Argonaute-family proteins play crucial roles in small-RNA-mediated gene regulation. In Drosophila, previous studies have demonstrated that Piwi, one member of the PIWI subfamily of Argonaute proteins, plays an essential role in regulating the fate of germline stem cells (GSCs). However, whether other Argonaute proteins also play similar roles remains elusive. Here, we show that overexpression of Argonaute 1 (AGO1) protein, another subfamily (AGO) of the Argonaute proteins, leads to GSC overproliferation, whereas loss of Ago1 results in the loss of GSCs. Combined with germline clonal analyses of Ago1, these findings strongly support the argument that Ago1 plays an essential and intrinsic role in the maintenance of GSCs. In contrast to previous observations of Piwi function in the maintenance of GSCs, we show that AGO1 is not required for bag of marbles (bam) silencing and probably acts downstream or parallel of bam in the regulation of GSC fate. Given that AGO1 serves as a key component of the miRNA pathway, we propose that an AGO1-dependent miRNA pathway probably plays an instructive role in repressing GSC/cystoblast differentiation.