Emergence of Triazole Resistance in Aspergillus fumigatus Exposed to Paclobutrazol
Emergence of Triazole Resistance in Aspergillus fumigatus Exposed to Paclobutrazol
复制标题
多效唑烟曲霉三唑耐药性的出现
DOI：
10.1021/acs.jafc.1c05396
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发表时间：
2021-12-17
影响因子：
6.1
通讯作者：
Yu, Yunlong
中科院分区：
文献类型：
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作者：
Yu, Sumei;Wang, Yingnan;Yu, Yunlong
作为一个全球性的健康问题，烟曲霉对三氮唑的耐药性来源已经引起了人们的广泛关注。本研究旨在探讨三氮唑植物调节剂多效唑是否会对烟曲霉产生三唑抗性。结果表明，从液体和土壤中分离到2株具有遗传稳定性的三氮唑抗性菌株。Cyp51A、cyp51B、AtrF、cdr1B、AfuMDR1、AfuMDR2和AfuMDR4基因的上调在这些耐药株中起重要作用。烟曲霉菌对三氮唑的抗性与多效唑浓度和暴露时间的选择压力有关。这些结果表明，多效唑的应用可能导致烟曲霉菌对三氮唑耐药的紧急状态，从而对侵袭性曲霉病的治疗具有潜在的风险。
As a global health problem, the source of triazole resistance in Aspergillus fumigatus has gained much attention. This study was conducted to explore whether the triazole plant regulator paclobutrazol could evolve triazole resistance in A. fumigatus. The results indicated that two triazole-resistant strains with hereditary stability were isolated from liquid medium and soil. The upregulation of cyp51A, cyp51B, AtrF, cdr1B, AfuMDR1, AfuMDR2, and AfuMDR4 played an important role in these resistant strains. The triazole-resistance in A. fumigatus could depend on the selective pressure of paclobutrazol concentration and exposure time. These results indicate that the application of paclobutrazol may result in the emergency of triazole resistance in A. fumigatus and thus have a potential risk for the treatment of invasive aspergillosis.