MICROMOLAR PROTEIN CONCENTRATIONS AND METALLOPROTEIN STOICHIOMETRIES OBTAINED BY INDUCTIVELY COUPLED PLASMA ATOMIC EMISSION SPECTROMETRIC DETERMINATION OF SULFUR
MICROMOLAR PROTEIN CONCENTRATIONS AND METALLOPROTEIN STOICHIOMETRIES OBTAINED BY INDUCTIVELY COUPLED PLASMA ATOMIC EMISSION SPECTROMETRIC DETERMINATION OF SULFUR
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DOI：
10.1021/ac00175a008
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发表时间：
1988-12-15
影响因子：
7.4
通讯作者：
BELL, JU
中科院分区：
文献类型：
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作者：
BONGERS, J;WALTON, CD;BELL, JU
用电感耦合等离子体原子发射光谱法（ICP-AES）测定了几种已知硫含量的蛋白质微摩尔溶液中硫的浓度。将这些值与通过使用分光光度测量和基于各种常规蛋白质测定方法的公布的比吸光系数和摩尔吸光系数获得的浓度进行比较。这两组值非常一致，表明硫的ICP-AES是测定微克量蛋白质的准确方法。6次重复测量数据集所得平均值的标准偏差在2%范围内。稀释的缓冲蛋白质溶液可直接泵入雾化器;不需要样品消化和其他特殊样品制备。它也证明了几种金属蛋白质，多元素ICP-AES是一种很好的手段，确定结合金属离子的化学计量，因为蛋白质和金属测定可以快速进行单一样品。
The concentrations of several micromolar solutions of proteins with known sulfur contents were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) of sulfur in the vacuum ultraviolet. These values are compared to concentrations obtained by using spectrophotometric measurements and published specific and molar absorptivities based on various conventional methods of protein determination. The two sets of values are in close agreement, indicating that ICP-AES of sulfur is an accurate means of determining microgram quantities of proteins. Standard deviations of within 2% of the mean values obtained for data sets of six replicate measurements. Dilute buffered protein solutions may be directly pumped into the nebulizer; sample digestion and other special sample preparations are not necessary. It is also demonstrated for several metalloproteins that multielement ICP-AES is an excellent means of determining stoichiometries of bound metal ions as both protein and metal assays may be rapidly performed on a single sample.