The effect of a bacteriophage on diversification of the opportunistic bacterial pathogen, Pseudomonas aeruginosa
The effect of a bacteriophage on diversification of the opportunistic bacterial pathogen, Pseudomonas aeruginosa
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DOI：
10.1098/rspb.2005.3086
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发表时间：
2005-07-07
影响因子：
4.7
通讯作者：
Rainey, PB
中科院分区：
文献类型：
--
作者：
Brockhurst, MA;Buckling, A;Rainey, PB
铜绿假单胞菌是一种机会性人类病原体，其定殖于囊性纤维化（CF）患者的肺。CF肺通常包含多种铜绿假单胞菌表型，其中一些可能导致感染的持续性，但多样性的原因尚不清楚。虽然肺环境的生态异质性和治疗方案是可能的因素，但不能排除寄生噬菌体的作用。寄生虫已被牵连作为一个关键的生态变量，推动宿主种群的多样性的演变。PP 7驱动铜绿假单胞菌宿主种群的形态多样化周期，这是由于与大的弥漫性菌落形态细菌共存的小粗糙菌落变体的从头进化。在不存在噬菌体的情况下，细菌仅显示野生型的大的弥散菌落形态。进一步的分析显示，有两种不同类型的耐药细菌;它们具有非常不同的生态表型，但每种都有耐药性的代价。
Pseudomonas aeruginosa is an opportunistic human pathogen that colonizes the lungs of cystic fibrosis (CF) patients. CF lungs often contain a diverse range of P aeruginosa phenotypes, some of which are likely to contribute to the persistence of infection, yet the causes of diversity are unclear. While the ecological heterogeneity of the lung environment and therapeutic regimes are probable factors, a role for parasitic bacteriophage cannot be ruled out. Parasites have been implicated as a key ecological variable driving the evolution of diversity in host populations. PP7 drove cycles of morphological diversification in host populations of P aeruginosa due to the de novo evolution of small-rough colony variants that coexisted with large diffuse colony morph bacteria. In the absence of phage, bacteria only displayed the large diffuse colony morphology of the wild-type. Further assays revealed there to be two distinct types of resistant bacteria; these had very different ecological phenotypes, yet each carried a cost of resistance.