Extracellular polymeric substances of biofilms: Suffering from an identity crisis
Extracellular polymeric substances of biofilms: Suffering from an identity crisis
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DOI：
10.1016/j.watres.2018.11.020
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发表时间：
2019-03-15
期刊：
影响因子：
12.8
通讯作者：
Lin, Yuemei
中科院分区：
文献类型：
--
作者：
Seviour, Thomas;Derlon, Nicolas;Lin, Yuemei
微生物生物膜可以是一系列新出现的社会问题的原因和治疗方法，包括抗菌素耐受性，水卫生，水资源短缺和污染。负责生物膜的建立和功能的胞外聚合物（EPS）的身份知之甚少。缺乏关于EPS的化学和物理特性的信息限制了合理设计生物膜过程的潜力，并阻碍了水和废水部门朝着循环经济和资源回收的方向取得进展。在这里，提出了一个多学科的路线图，以解决这一EPS身份危机。这涉及改进的EPS提取和表征方法，模型生物膜和全规模生物膜系统之间的交叉引用，以及与基因组学，蛋白质组学和糖组学相结合的原位技术（例如显微镜）对分离的EPS的功能描述。目前的提取和分光光度法表征方法，往往是基于不损害微生物细胞的完整性的原则，应严格评估，更全面的方法回收和表征的EPS需要开发。(C)2018爱思唯尔有限公司版权所有
Microbial biofilms can be both cause and cure to a range of emerging societal problems including antimicrobial tolerance, water sanitation, water scarcity and pollution. The identities of extracellular polymeric substances (EPS) responsible for the establishment and function of biofilms are poorly understood. The lack of information on the chemical and physical identities of EPS limits the potential to rationally engineer biofilm processes, and impedes progress within the water and wastewater sector towards a circular economy and resource recovery. Here, a multidisciplinary roadmap for addressing this EPS identity crisis is proposed. This involves improved EPS extraction and characterization methodologies, cross-referencing between model biofilms and full-scale biofilm systems, and functional description of isolated EPS with in situ techniques (e.g. microscopy) coupled with genomics, proteomics and glycomics. The current extraction and spectrophotometric characterization methods, often based on the principle not to compromise the integrity of the microbial cells, should be critically assessed, and more comprehensive methods for recovery and characterization of EPS need to be developed. (C) 2018 Elsevier Ltd. All rights reserved.