Partial characterization and anti-tumor activity of an acidic polysaccharide from Gracilaria lemaneiformis
Partial characterization and anti-tumor activity of an acidic polysaccharide from Gracilaria lemaneiformis
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龙须菜酸性多糖的部分表征和抗肿瘤活性
DOI：
10.1016/j.carbpol.2012.02.014
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发表时间：
2012-05-16
影响因子：
11.2
通讯作者：
Liu, Anjun
中科院分区：
文献类型：
--
作者：
Fan, Yanli;Wang, Wenhang;Liu, Anjun
从龙须菜中分离得到一种酸性多糖(GLSPs)，其碳水化合物含量为72.06%，硫酸盐含量为6.13%。 GLSPs的FTIR分析表明，在1259和804 cm(-1)处出现两个典型吸收，分别归因于S=O和C-O-S键的拉伸。 HPGPC显示GLSPs的分子量主要分布在1.37 x 10(6) Da。 GC分析表明GLSPs主要由半乳糖组成，还含有少量鼠李糖、阿拉伯糖、木糖和甘露糖。此外，在移植 H22 肝癌细胞的 ICR 小鼠中评估了 GLSP 的抗肿瘤和免疫调节作用。结果表明，GLSPs能显着抑制荷瘤小鼠肿瘤的生长，促进脾细胞增殖和巨噬细胞吞噬作用，并提高血液中IL-2和CD8(+) T细胞的水平。结果表明，GLSPs表现出显着的抗肿瘤和免疫调节活性，可能为健康食品和临床药物提供潜在的材料。 (C) 2012 Elsevier Ltd. 保留所有权利。
An acidic polysaccharide (GLSPs) with carbohydrate content of 72.06% and sulfate content of 6.13% was isolated from Gracilaria lemaneiformis. FTIR analysis of GLSPs showed that two typical absorptions appeared at 1259 and 804 cm(-1) were assigned to S=O and C-O-S bonds stretching respectively. HPGPC showed the molecular weight of GLSPs was mainly distributed at 1.37 x 10(6) Da. GC analysis showed GLSPs was chiefly composed of galactose, and contained a small amount of rhamnose, arabinose, xylose and mannose. Moreover, the anti-tumor and immunomodulatory effects of GLSPs were evaluated in ICR mice transplanted H22 hepatoma cells. It was shown that GLSPs significantly inhibited the growth of tumor, promoted splenocytes proliferation and macrophage phagocytosis, and increased the level of IL-2 and CD8(+) T cells in blood of tumor-bearing mice. The results suggested that GLSPs displayed remarkable anti-tumor and immunomodulatory activities, and it may provide a potential material of healthy food and clinical medicines. (C) 2012 Elsevier Ltd. All rights reserved.