Endothelium removal does not abolish angiotensin converting enzyme activity from the mesenteric arterial bed of the rat.
Endothelium removal does not abolish angiotensin converting enzyme activity from the mesenteric arterial bed of the rat.
复制标题
内皮去除不会消除大鼠肠系膜动脉床的血管紧张素转化酶活性。
DOI：
10.1016/0006-2952(90)90506-g
复制
发表时间：
1990
影响因子：
5.8
通讯作者：
Maragoudakis,ME
中科院分区：
文献类型：
--
作者：
Pipili-Synetos,E;Sideri,E;Catravas,JD;Maragoudakis,ME
材料和方法灌注肠系膜动脉床。用乙醚麻醉20-25周龄、体重250-350g的Wistar-Kyoto大鼠。随后，如 McGregor 所描述的那样，对肠系膜动脉床进行插管并移除[8]。然后将制剂转移到恒温控制的有机玻璃床上，保持在37英寸，并通过血管灌注以下成分的含氧（95％Or，5％COr）McEwen溶液（mM：NaCl 130，NaHCO，25，KCl 5.6，CaClr 2.2，NaHPO 1.2，葡萄糖11和蔗糖13。灌注速率为1 mL/min，通过蠕动泵（LKB microperpex 2132）实现。床的血管体积约为 0.25 mL。内皮去除通过输注脱氧胆酸钠（1 mg/mL 盐水）40 秒来去除内皮细胞（通过乙酰胆碱（0.03-3.0）的松弛作用来检查是否成功去除内皮细胞。 pg，推注）在用去甲肾上腺素（NA）（1 pg/mL）预收缩的MAB上进行，并在用甲苯胺蓝和苏木精-伊红染色后进行组织学检查。
Materials and MethodsThe perfused mesenteric arterial bed. Wistar-Kyoto rats20-25 weeks old weighing 250-350 g were anesthetized with ethyl ether. The mesenteric arterial bed was subsequently cannulated and removed, as described by McGregor [8]. The preparation was then transferred to a thermostatically controlled perspex bed, maintained at 37” and perfused through the vessels with oxygenated (95% Or, 5% COr) McEwen’s solution of the following composition (mM: NaCl 130, NaHCO, 25, KC1 5.6, CaClr 2.2, NaHPO., 1.2, glucose 11 and sucrose 13. The perfusion rate was 1 mL/min and was achieved by means of a peristaltic pump (LKB microperpex 2132). The vascular volume of the bed was approximately 0.25 mL.Endothelium removal. The endothelium was removed by infusion of sodium deoxycholate (1 mg/mL in saline) for 40sec [9]. The successful removal of endothelium was checked by the lack of relaxant effect of acetylcholine (0.03-3.0 pg, bolus injection) on the MAB precontracted with noradrenaline (NA)(1 pg/mL) and by histological examination following staining with toluidine blue and hematoxylin-eosin.