Development of a monocyte/macrophage-like cell line, RTS11, from rainbow trout spleen
Development of a monocyte/macrophage-like cell line, RTS11, from rainbow trout spleen
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DOI：
10.1006/fsim.1998.0153
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发表时间：
1998-08-01
影响因子：
4.7
通讯作者：
Bols, NC
中科院分区：
文献类型：
--
作者：
Ganassin, RC;Bols, NC
一个新的虹鳟鱼细胞系，RTS 11，自发产生的长期造血培养的脾脏。对于常规传代培养，RTS 11需要20-30%胎牛血清、高细胞密度和来自先前培养物的条件培养基。通过连续传代，RTS 11培养物在形成大多数细胞的小的非粘附细胞（称为圆形细胞）和少数较大的颗粒细胞之间保持平衡，所述颗粒细胞是粘附的或在由圆形细胞簇包围的悬浮液中。大颗粒细胞似乎是巨噬细胞，并被称为巨噬细胞样细胞，因为它们是吞噬细胞，摄取二碘乙酰化低密度脂蛋白和吖啶橙子并对非特异性酯酶染色，而圆形细胞似乎处于巨噬细胞发育的早期阶段。RTS 11调节的培养基也含有溶菌酶样活性，这是它们属于巨噬细胞谱系的另一个指示。在增殖测定中，非贴壁细胞受到RTS 11条件培养基和脂多糖的强烈刺激。虹鳟头肾白细胞对RTS 11条件培养基也显示出阳性增殖反应。因此，RTS 11细胞似乎产生并响应自分泌生长因子。(C)北京：科学出版社.
A new rainbow trout cell line, RTS11, arose spontaneously from a long-term haemopoietic culture of spleen. For routine subculture, RTS11 requires 20-30% fetal bovine serum, a high cell density, and conditioned medium from previous cultures. Through successive passages, RTS11 cultures maintain a balance between small, non-adherent cells that form the majority of cells (referred to as round cells) and a few larger, granular cells that are either adherent or in suspension surrounded by clusters of round cells. The large granular cells appear to be macrophages, and are referred to as macrophagelike cells, because they are phagocytic, take up DiI-acetylated low density lipoprotein and acridine orange and stain for non-specific esterase, whereas the round cells appear to be at an earlier stage of macrophage development. Medium conditioned by RTS11 also contains lysozyme-like activity, an additional indication that they are of the macrophage lineage. In proliferation assays, the non-adherent cells are stimulated strongly by RTS11 conditioned medium and by lipopolysaccharide. Rainbow trout head kidney leucocytes also show a positive proliferative response to RTS11 conditioned medium. Therefore, RTS11 cells appear to both produce and respond to an autocrine growth factor(s). (C) 1998 Academic Press.