Genomic organization and promoter function of the human thyrotropin-releasing hormone receptor gene
Genomic organization and promoter function of the human thyrotropin-releasing hormone receptor gene
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DOI：
10.1074/jbc.271.36.22183
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发表时间：
1996-09-06
影响因子：
4.8
通讯作者：
Mori, M
中科院分区：
文献类型：
--
作者：
Iwasaki, T;Yamada, M;Mori, M
我们分离并鉴定了人促甲状腺激素释放激素受体基因，该基因全长30kb，包含3个外显子和2个内含子，内含子1存在于5‘-非翻译区，内含子2长度超过25kb，在推测的第六跨膜结构域开始之前中断了编码区。外显子3编码编码区的其余部分和整个3‘-非翻译区。3‘-侧翼区含有4个潜在的多聚腺苷酸化信号，3’-末端快速扩增研究表明，只有终止密码子下游2076个碱基对的信号在垂体前叶起作用，引物延伸和锚定-聚合酶链式反应研究表明，在翻译起始点上游的344个碱基对上有一个转录起始点，启动子区域在适当的位置既不包含TATA盒，也不包含CAAT盒。瞬时转染研究表明，启动子在GH4C1细胞中具有显著的活性，转录起始点-338--933bp之间的区域起负调控作用。对促甲状腺激素释放激素(TRH)受体基因的基因组结构和启动子区域的了解将有助于进一步研究TRH受体可能的紊乱，并有助于阐明人类TRH受体基因的转录调控。
We isolated and characterized the gene for the human thyrotropin-releasing hormone receptor, The gene spanned more than 30 kilobases and contained three exons and two introns, Intron 1 exists in the 5'-untranslated region, and intron 2 is more than 25 kilobases in length which interrupts the coding region before the beginning of the putative sixth transmembrane domain. Exon 3 encodes the rest of the coding region and the entire 3'-untranslated region. The 3'-flanking region contains four potential polyadenylation signals, and 3'-rapid amplification of cDNA ends studies showed that only a signal at 2076 base pairs downstream of the stop codon was functional in the anterior pituitary, Primer extension and anchor-polymerase chain reaction studies indicated a transcriptional start site at 344 base pairs upstream of the translational start site, The promoter region does not contain either a TATA box or a CAAT box in the appropriate location, Transient transfection study revealed significant activity of the promoter in GH4C1 cells, and the region between -338 and -933 bp from the transcriptional start site worked as a negative regulator, Knowledge of the genomic organization and the promoter region of thyrotropin-releasing hormone (TRH) receptor gene will allow further studies of possible disorders of the TRH receptor, as well as facilitate elucidation of transcriptional control of the human TRH receptor gene.