Surface plasmon resonance sensing with adjustable sensitivity based on a flexible liquid core coupling unit
Surface plasmon resonance sensing with adjustable sensitivity based on a flexible liquid core coupling unit
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基于柔性液芯耦合单元的可调灵敏度表面等离子体共振传感
DOI：
10.1016/j.talanta.2018.03.040
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发表时间：
2018
期刊：
影响因子：
6.1
通讯作者：
Chen Yi
中科院分区：
文献类型：
--
作者：
Xu Jiying;Chen Yi
表面等离子体共振（SPR）的信号放大方法主要是样品选择性放大。提出了一种灵敏度可调、具有普遍适用性的液芯耦合SPR传感方法。设计了一种液芯耦合单元，并以此为基础设计制作了一种波长解调型SPR传感器，该传感器可以利用传统的芯片，通过巧妙的设计，通过改变液芯折射率来调节传感器的灵敏度。实验结果表明，当液芯折射率从1.5174降低到1.4502时，灵敏度可提高7.8倍。通过癌胚抗原（CEA）的检测，证实了该方法的真实的适用性，早期结肠癌患者的CEA信号强度明显高于健康人近18倍。它表现出足够的灵敏度，并进一步考虑到其简单性，避免了麻烦的分析过程和/或芯片制备过程，并可扩展到SPR成像，所提出的方法将有望用于医疗诊断，食品安全，环境监测等。
The signal amplification methods of surface plasmon resonance (SPR) are mainly with sample selectivity. Herein proposed is a liquid core coupling SPR sensing method with adjustable sensitivity and universal applicability. A liquid core coupling unit and based on which a wavelength interrogation SPR sensor have been designed and constructed, in which a conventional chip could be used with an ingenious design and the sensitivity can be adjusted by changing the liquid core refractive index. The experimental data revealed that the sensitivity can be enhanced for 7.8 times by decreasing the liquid core refractive index from 1.5174 to 1.4502. The real applicability was validated by determination of carcinoembryonic antigen (CEA) with the signal intensity of the early stage colon cancer patient clearly up-regulated for nearly 18 folds compared with the healthy. It exhibited an adequate sensitivity, and further considering its simplicity avoiding the troublesome analysis process and/or chip preparing procedure, and extendibility to SPR imaging, the proposed method would be promising for medical diagnostics, food safety, environmental monitoring, and so on.