Knockdown Resistance-Associated Mutations Dominate Populations of the Common Bed Bug (Hemiptera: Cimicidae) Across the South Central United States
Knockdown Resistance-Associated Mutations Dominate Populations of the Common Bed Bug (Hemiptera: Cimicidae) Across the South Central United States
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DOI：
10.1093/jme/tjz105
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发表时间：
2019-11-01
影响因子：
2.1
通讯作者：
Booth, Warren
中科院分区：
文献类型：
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作者：
Holleman, Jeffrey G.;Robison, Grant A.;Booth, Warren
尽管人们知道臭虫Cimex lectularius L.(半翅目：Cimicidae)中存在导致抗药性的突变，但在美国国内，很少有研究涉及这些突变的分布和频率。在美国，研究集中在东海岸和中西部的收集，记录了电压门控钠通道阿尔法亚单位基因中的两个突变(V419L和L925I)的发生，表明与对拟除虫菊酯的击倒抗性(Kdr)有关。这里，V419L和L925I位点变异的分布和频率是从俄克拉荷马州及其邻近州的感染样本中报告的。此外，还注意到存在一种以前在美国没有记录的突变(I935F)。虽然这种突变在美国是新奇的，但此前在澳大利亚和东半球的人群中也有过这种突变的报道。在这三个地点都没有发现滋生野生型个体的寄生虫，因此它们都很容易受到感染。相反，有接近21%的人在L925I位点存在耐药突变(单倍型B)，类似77%的人在V419L和L925I位点同时存在突变(单倍型C)，2%的人在L936F位点存在突变(单倍型A(B))。单倍型C的高频率与美国以前的研究一致，与旧世界和澳大利亚的研究形成鲜明对比。这里提供的数据提供了对美国中南部地区当代发生的与kdr相关的杀虫剂耐药性的洞察，该地区以前没有数据。这些数据表明，新世界和旧世界/澳大利亚的感染可能来自不同的来源。
Despite awareness of the mutations conferring insecticide resistance in the bed bug, Cimex lectularius L. (Hemiptera: Cimicidae), within the United States few studies address the distribution and frequency of these. Within the United States, studies have focused on collections made along the East Coast and Midwest, documenting the occurrence of two mutations (V419L and L925I) within the voltage-gated sodium channel alpha-subunit gene shown to be associated with knockdown resistance (kdr) to pyrethroids. Here, the distribution and frequency of the V419L and L925I site variants is reported from infestations sampled within Oklahoma and its immediately adjacent states. Additionally, the presence of a mutation previously undocumented in the United States (I935F) is noted. While novel in the United States, this mutation has previously been reported in Australian and Old World populations. No infestations were found to harbor wild-type individuals, and hence susceptible, at each of the three sites. Instead, similar to 21% were found to possess the resistant mutation at the L925I site (haplotype B), similar to 77% had mutations at both the V419L and L925I sites (haplotype C), and 2% possessed the mutation at the L936F site (haplotype A(b)). The high frequency of haplotype C corresponds to previous studies in the United States, and contrasts dramatically with those of the Old World and Australia. The data presented here provide insight into the contemporary occurrence of kdr-associated insecticide resistance in the South Central United States, a region for which data have previously been absent. These data suggest that New World and Old World/Australian infestations are likely to have originated from different origins.