Identification of gene products expressed in the developing chick visual system: characterization of a middle-molecular-weight neurofilament cDNA.
Identification of gene products expressed in the developing chick visual system: characterization of a middle-molecular-weight neurofilament cDNA.
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发育中的雏鸡视觉系统中表达的基因产物的鉴定：中等分子量神经丝 cDNA 的表征。
DOI：
10.1101/gad.1.7.699
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发表时间：
1987
影响因子：
10.5
通讯作者：
H. Betz
中科院分区：
文献类型：
--
作者：
D. Zopf;I. Hermans‐Borgmeyer;E. Gundelfinger;H. Betz
从鸡视叶组织的poly（A）+ RNA构建了一个cDNA文库，该文库富集了从胚胎第7天到孵化后第1天积累的转录物。从这个文库中，分离出三个重组体，它们对应于在视网膜顶盖系统中突触发生的主要时期出现的神经mRNA。这些重组体之一（OZ 11）被确定为中分子量神经丝（NF-M）的cDNA，连同两个重叠的克隆，编码部分杆和整个尾区的NF-M。原位杂交显示NF-M mRNA在鸡中枢神经系统的区域中高度表达，该区域包含多极和/或长投射神经元。
A cDNA library enriched for transcripts that accumulate from embryonic day 7 until day 1 posthatching was constructed from poly(A)+ RNA of chick optic lobe tissue. From this library, three recombinants were isolated that correspond to neural mRNAs appearing during the major period of synaptogenesis in the retinotectal system. One of these recombinants (OZ 11) was identified as a middle-molecular weight neurofilament (NF-M) cDNA which, together with two overlapping clones, encodes part of the rod and the entire tail region of NF-M. In situ hybridization revealed NF-M mRNA to be highly expressed in regions of the chick central nervous system, which contain multipolar and/or long-projection neurons.