MUTATIONS IN THE PAR GENES OF CAENORHABDITIS-ELEGANS AFFECT CYTOPLASMIC REORGANIZATION DURING THE 1ST CELL-CYCLE
MUTATIONS IN THE PAR GENES OF CAENORHABDITIS-ELEGANS AFFECT CYTOPLASMIC REORGANIZATION DURING THE 1ST CELL-CYCLE
复制标题
DOI：
10.1016/0012-1606(90)90164-e
复制
发表时间：
1990-11-01
影响因子：
2.7
通讯作者：
KEMPHUES, K
中科院分区：
文献类型：
--
作者：
KIRBY, C;KUSCH, M;KEMPHUES, K
在线虫胚胎的第一个细胞周期期间，细胞质发生了戏剧性的重组。我们在此介绍对野生型胚胎和标准突变胚胎重组过程中的一些事件进行定量研究的结果。 par突变定义了早期胚胎细胞质定位所需的一组基因。我们证明 par 突变会导致重组的几个事件中的缺陷。我们研究的所有四个 por 基因的突变都会导致皮质微丝的假分裂和不对称重新分布缺陷。此外，一些 par 突变影响细胞质的流动、原核的迁移和胚胎的不对称缩短。我们认为 par 基因的主要功能可能是协调细胞质的初始重组。
A dramatic reorganization of cytoplasm occurs during the first cell cycle in embryos of the nematode, Caenorhabditis elegans. We present here the results of a quantitative study of some of the events during this reorganization in wild-type embryos and in par mutant embryos. The par mutations define a set of genes required for cytoplasmic localization in early embryos. We show that par mutations lead to defects in several events of the reorganization. Mutations in all four of the por genes we studied lead to defects in pseudocleavage and asymmetric redistribution of cortical microfilaments. In addition, some of the par mutations affect streaming of cytoplasm, migration of the pronuclei, and asymmetric shortening of the embryo. We propose that the major function of the par genes might be to orchestrate this initial reorganization of cytoplasm.