FERTILIZATION ABNORMALITIES IN HUMAN INVITRO FERTILIZATION
FERTILIZATION ABNORMALITIES IN HUMAN INVITRO FERTILIZATION
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DOI：
10.1093/humrep/7.suppl_1.89
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发表时间：
1992-06-01
影响因子：
6.1
通讯作者：
CROZET, N
中科院分区：
文献类型：
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作者：
PLACHOT, M;CROZET, N
对受精异常(精子染色体过早凝聚、三倍体、单倍体)进行分析，以确定其来源。9%的未受精卵母细胞中会出现PCC，这似乎是卵母细胞激活失败的结果，导致细胞质染色体浓缩因子的持续存在，导致精子核过早发生染色体浓缩。这种异常似乎与核和/或细胞质成熟不完全有关。三倍体受精卵(占受精卵母细胞的6.5%)表现出原始的分裂类型：其中一半分裂为3和6个细胞，而二倍体受精卵同时分裂为2个细胞和4个细胞。细胞学研究，同时使用抗微管蛋白抗体和Hoechst染料，使我们能够证明它们通过三极纺锤体分裂成3个细胞。三倍体似乎与检查的16个临床或生物学参数中的4个有关：精液来源(新鲜或冷冻)、刺激处理类型、回收的卵母细胞数量和胚胎形态。单倍体卵子(占受精卵母细胞的1.6%)是孤雌激活的结果。回收的卵母细胞数和三倍体合子数与卵母细胞激活的发生密切相关。这些数据表明，卵泡招募的增加降低了卵母细胞的整体质量和成熟度，导致受精率总体为9%，受精率下降。
Fertilization abnormalities (premature chromosome condensation of spermatozoa (PCC), triploidy, haploidy) were analysed in order to determine their origin. PCC occurs in 9% of unfertilized oocytes and seems to be the consequence of a failure of oocyte activation, leading to the continuing presence of cytoplasmic chromosome-condensing factors, causing the sperm nucleus to undergo chromosome condensation prematurely. This anomaly appears to be related to incomplete nuclear and/or cytoplasmic maturation. Triploid zygotes (6.5% of fertilized oocytes) display an original type of division: half of them divide into 3 and 6 cells, whereas at the same time diploid zygotes divide into 2 and then 4 cells. A cytological study, using both antitubulin antibodies and Hoechst dye, allowed us to demonstrate that they divide into 3 cells by means of a tripolar spindle. Triploidy seems to be correlated with four of 16 clinical or biological parameters examined: semen origin (fresh or frozen), type of stimulation treatment, number of oocytes recovered and embryo morphology. Haploid eggs (1.6% of inseminated oocytes) result from parthenogenetic activation. A correlation was found between a high number of recovered oocytes and triploid zygotes, and the occurrence of oocyte activation. These data show that increasing follicular recruitment decreases the overall oocyte quality and maturity leading to an overall 9% with impaired fortilization.