The FvPHR1 transcription factor control phosphate homeostasis by transcriptionally regulating miR399a in woodland strawberry
The FvPHR1 transcription factor control phosphate homeostasis by transcriptionally regulating miR399a in woodland strawberry
复制标题
FvPHR1转录因子通过转录调节林地草莓中的miR399a来控制磷酸盐稳态
DOI：
10.1016/j.plantsci.2018.12.025
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发表时间：
2019
期刊：
影响因子：
5.2
通讯作者：
Zhihong Zhang
中科院分区：
文献类型：
--
作者：
Yan Wang;Feng Zhang;Weixu Cui;Keqin Chen;Rui Zhao;Zhihong Zhang
植物为了适应低磷环境，已经进化出磷饥饿反应。PHR 1-miR 399-PHO 2模块对磷缺乏适应性反应的调节在拟南芥中已经得到了很好的研究，但在草莓中还没有。转录因子PHR 1作为磷饥饿信号的中心调节因子已在一些植物中被发现。然而，PHR 1同源物在草莓中的功能仍然未知。本研究在野生草莓（Fragaria vesca）基因组中鉴定了13个MYB-CC基因，并对FvPHR 1基因进行了分析。FvPHR 1含有MYB结构域和卷曲螺旋（CC）结构域，定位于细胞核。FvPHR 1也具有反式激活能力。FvPHR 1基因过表达的林地草莓叶片中磷含量比野生型草莓叶片中磷含量提高了1.38 ~ 1.78倍。FvPHR 1与FvMIR 399 a启动子结合，对FvMIR 399 a的表达具有正调控作用。这些结果表明，PHR 1-miR 399模块参与了林地草莓磷信号通路和磷稳态的调控。
Plants have evolved phosphate (Pi) starvation response to adapt the low-Pi environment. The regulation of adaptive responses to phosphorus deficiency by the PHR1-miR399-PHO2 module has been well studied inArabidopsis thalianabut not in strawberry. Transcription factor PHR1 as the central regulator in the Pi starvation signaling has been revealed in a few plant species. However, the function of PHR1 homologues in strawberry is still unknown. In this study, a total of 13 MYB-CC genes were identified in the woodland strawberry (Fragaria vesca) genome and theFvPHR1gene was characterized. FvPHR1 contains MYB domain and coiled–coil (CC) domain and is localized in the nucleus. FvPHR1 also exhibits trans-activation ability. Furthermore, the P content in leaves ofFvPHR1-overexpressing woodland strawberries was significantly increased by 1.38-fold to 1.78-fold compared with that in the wild type. FvPHR1 was also demonstrated to directly bind to theFvMIR399apromoter and positively regulate the expression of FvmiR399a in woodland strawberry. These results showed that PHR1-miR399 module is involved in the regulation of phosphate-signaling pathway and phosphate homeostasis in woodland strawberry.