OP-1 enhances dendritic growth from cerebral cortical neurons in vitro
OP-1 enhances dendritic growth from cerebral cortical neurons in vitro
复制标题
DOI：
10.1006/exnr.1999.7194
复制
发表时间：
1999-11-01
影响因子：
5.3
通讯作者：
Charette, M
中科院分区：
文献类型：
--
作者：
Le Roux, P;Behar, S;Charette, M
成骨蛋白-1 (OP-1) 是转化生长因子-β (TGF-β) 超家族的成员，已被证明可以刺激培养物中交感神经元的树突生长。然而，尚不清楚OP-1是否影响中枢神经系统神经元的树突生长。因此，我们量化了在化学成分确定的培养基中体外生长的胚胎小鼠皮质神经元 (E 18) 的轴突以及初级、次级和总树突的生长。体外 3 天后，使用形态学和双重免疫标记（MAP2、NF-H）来鉴定皮质树突和轴突。在所有实验条件下，细胞形态、神经元存活和轴突长度相似。初生枝晶的数量也相似；然而，通过向培养基中添加OP-1，初级树突的长度以及次级树突的长度和数量显着增加。树突生长的增加是剂量依赖性的；向培养基中添加30-100ng/ml OP-1后观察到最大树突生长。当神经元暴露于 TGF-β 超家族的其他成员时，没有观察到树突生长的特异性支持。这些发现证明OP-1在体外选择性地增加大脑皮层神经元的树突生长。 (C) 1999 年学术出版社。
Osteogenic protein-1 (OP-l), a member of the transforming growth factor-beta (TGF-beta) superfamily, has been demonstrated to stimulate dendrite growth from sympathetic neurons in culture. However, it is not known whether OP-l affects dendrite growth from central nervous system neurons. Therefore we quantified axon and primary, secondary, and total dendritic growth from embryonic mouse cortical neurons (E 18) grown in vitro in a chemically defined medium. Morphology and double immunolabeling (MAP2, NF-H) were used to identify cortical dendrites and axons after 3 days in vitro. Cell morphology, neuron survival, and axon length were similar under all experimental conditions. The number of primary dendrites also was similar; however, the length of primary dendrites and the length and number of secondary dendrites were significantly increased by the addition of OP-l to the culture medium. This increase in dendrite growth was dose-dependent; maximal dendritic growth was observed after the addition of 30-100 ng/ml of OP-l to the culture medium. Specific support of dendrite growth was not observed when neurons were exposed to other members of the TGF-beta superfamily. These findings demonstrate that OP-l selectively increases dendrite growth from cerebral cortical neurons in vitro. (C) 1999 Academic Press.