Distribution of UDPglucuronosyltransferase in rat tissue.
Distribution of UDPglucuronosyltransferase in rat tissue.
复制标题
UDP葡萄糖醛酸基转移酶在大鼠组织中的分布。
DOI：
10.1073/pnas.82.9.2990
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发表时间：
1985
影响因子：
11.1
通讯作者：
Novikoff,AB
中科院分区：
文献类型：
--
作者：
Chowdhury,JR;Novikoff,PM;Chowdhury,NR;Novikoff,AB
UDPglucuronosyltransferase [UDPglucuronate beta-D-glucuronosyltransferase (receptor -unspecific), EC 2.4.1.17]是一组具有不同但部分重叠的底物特异性的酶。兔抗血清对一种纯化的大鼠肝脏UDPglucuronosyltransferase亚型具有特异性，并通过免疫扩散或免疫转印迹分析识别所有的转移酶亚型。对所有底物的转移酶活性由抗血清纯化的IgG从溶解的大鼠肝微粒体免疫吸收。纯化的IgG用于大鼠肝脏、空肠、肾脏和肾上腺的udp -葡萄糖醛酸糖基转移酶的免疫细胞化学定位。在肝脏中，UDPglucuronosyltransferase仅存在于肝细胞中，并均匀分布于肝小叶的各个区域。在空肠中，转移酶仅存在于上皮细胞中，并且从隐窝到绒毛尖端的浓度逐渐增加。在肾脏中，转移酶在近曲小管上皮细胞中浓度最高。肾上腺髓质细胞呈强免疫细胞化学染色；肾上腺皮质的肾小球带和网状带比束状带染色更强烈。光镜下，4个脏器的内质网和核膜中均有UDPglucuronosyltransferase；电镜下肝细胞证实了这一点。在线粒体、高尔基体、溶酶体、过氧化物酶体和质膜中未观察到转移酶，即使在各种底物特异性UDPglucuronosyltransferase活性被诱导3至4倍后也是如此。
UDPglucuronosyltransferase [UDPglucuronate beta-D-glucuronosyltransferase (acceptor-unspecific), EC 2.4.1.17] is a group of enzymes with distinct but partially overlapping substrate specificity. A rabbit antiserum raised against one purified rat liver UDPglycuronosyltransferase isoform was specific for UDPglucuronosyltransferase and recognized all transferase isoforms by immunodiffusion or immunotransblot analysis. The transferase activity toward all substrates was immunoabsorbed from solubilized rat liver microsomes by IgG purified from the antiserum. The purified IgG was used for immunocytochemical localization of UDP-glucuronosyltransferase in rat liver, jejunum, kidney, and adrenal gland. In the liver, UDPglucuronosyltransferase was present exclusively in hepatocytes and was uniformly distributed within all zones of the hepatic lobule. In the jejunum, the transferase was present exclusively in the epithelial cells and showed a progressive increase in concentration from the crypt to the villar tip. In the kidney, the greatest concentration of the transferase was observed in the epithelial cells of the proximal convoluted tubule. Adrenal medullary cells showed intense immunocytochemical staining; the zona glomerulosa and the zona reticularis of the adrenal cortex were more intensely stained than the zona fasciculata. By light microscopy, UDPglucuronosyltransferase was found in the endoplasmic reticulum and nuclear envelope of all the four organs; this was confirmed in the hepatocyte by electron microscopy. The transferase was not observed in mitochondria, Golgi apparatus, lysosomes, peroxisomes, and plasma membrane, even after 3- to 4-fold induction of various substrate-specific UDPglucuronosyltransferase activities.