Osteogenic and pH stimuli-responsive self-healing coating on biomedical Mg-1Ca alloy
Osteogenic and pH stimuli-responsive self-healing coating on biomedical Mg-1Ca alloy
复制标题
生物医用 Mg-1Ca 合金上的成骨和 pH 刺激响应自修复涂层
DOI：
10.1016/j.actbio.2019.05.027
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发表时间：
2019
影响因子：
9.7
通讯作者：
Zheng Yufeng
中科院分区：
文献类型：
--
作者：
Xiong Pan;Jia Zhaojun;Zhou Wenhao;Yan Jianglong;Wang Pei;Yuan Wei;Li Yangyang;Cheng Yan;Guan Zhenpeng;Zheng Yufeng
已经使用各种涂层来减缓生物医学镁合金的腐蚀速率。然而，这些涂层通常仅作为被动屏障。更希望赋予这种涂层以活性的、生物腐蚀响应的自修复能力。在本工作中，自修复涂层系统（表示为“丝-PA”）以氟化物预涂层（底部）、丝-植酸（PA）涂层（中间）和丝素蛋白涂层（顶部）的三明治结构的形式构建。在这里，PA负载在中间涂层作为缓蚀剂，利用其强大的螯合能力对溶解Mg 2+和Ca 2+离子。通过划痕试验和SVET试验评价了膜的自修复性能，通过浸泡试验和电化学测试评价了膜的耐腐蚀性能。结果表明，丝素PA对Mg-1Ca合金具有良好的自修复能力，并具有pH响应性，从而提高了Mg-1Ca合金的耐蚀性。使用MC 3 T3-E1细胞进一步评价了涂层系统的生物相容性和成骨活性，并且其在多种细胞行为中表现出有利的反应，即，粘附、扩散、增殖和分化。这些研究结果开辟了新的机会，在研究中的自我修复涂层的保护，以防止在生物医用镁合金。声明的SignificanceIn本研究中，一个自我修复涂层系统的pH刺激响应性和成骨活性的Mg-1Ca合金，通过集成丝素蛋白屏障涂层，丝纤维蛋白/植酸复合涂层，和氟化物预涂层。与传统的表面改性层相比，该涂层系统表现出令人感兴趣的自修复能力。此外，自修复能力提高了生物医用镁合金的耐腐蚀性，而涂层系统的有效成分赋予基体成骨活性。本工作为医用镁合金智能表面改性提供了新的思路。
Various coatings have been used to slow down the corrosion rate of biomedical magnesium alloys. However, these coatings usually act only as passive barriers. It is much more desirable to endow such coatings with active, biocorrosion-responsive self-repairing capacity. In the present work, a self-healing coating system (denoted as “silk-PA”) was constructed in the form of a sandwich architecture of fluoride precoating (bottom), silk-phytic acid (PA) coating (middle), and silk fibroin coating (top). Here, PA was loaded in the middle coating as a corrosion inhibitor by harnessing its strong chelating ability toward dissolving Mg2+and Ca2+ions. The self-healing property was evaluated by scratch and SVET tests, and the corrosion resistance was evaluated byin vitroimmersion and electrochemical measurements. The results showed that the silk-PA manifested intriguing self-healing capacity with pH responsiveness, hence profiting the corrosion resistance of the Mg-1Ca alloy. The biocompatibility and osteogenic activity of the coating system were further evaluated using MC3T3-E1 cells, and it demonstrated favorable responses in multiple cellular behaviors, i.e., adherence, spreading, proliferation, and differentiation. These findings open new opportunities in the study of self-healing coatings for protection against corrosion in biomedical Mg alloys.Statement of SignificanceIn the present study, a self-healing coating system with pH stimuli-responsiveness and osteogenic activity was fabricated on Mg-1Ca alloy by integrating a silk fibroin barrier coating, a silk fibrin/phytic acid composite coating, and a fluoride precoating. This coating system demonstrated interesting self-healing ability as compared to traditional surface modification layers. Furthermore, the self-healing ability enhanced the corrosion resistance of biomedical magnesium alloys, while effective compositions of the coating system endowed the substrate with osteogenic activity. This work provides some new insights into smart surface modification for biomedical Mg alloys.