ENHANCED LEG EXERCISE ENDURANCE WITH A HIGH-CARBOHYDRATE DIET AND DIHYDROXYACETONE AND PYRUVATE
ENHANCED LEG EXERCISE ENDURANCE WITH A HIGH-CARBOHYDRATE DIET AND DIHYDROXYACETONE AND PYRUVATE
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DOI：
10.1152/jappl.1990.69.5.1651
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发表时间：
1990-11-01
影响因子：
3.3
通讯作者：
GOSS, FL
中科院分区：
文献类型：
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作者：
STANKO, RT;ROBERTSON, RJ;GOSS, FL
研究了饮食补充二羟丙酮和丙酮酸(DHAP)对8名男性(20~30岁)腿部运动时代谢反应和耐力的影响。在运动前7天，摄入高碳水化合物饮食(70%碳水化合物、18%蛋白质、12%脂肪；35千卡/千克体重)。100克多糖(安慰剂)或二羟基丙酮和丙酮酸(治疗，3：1)取代了部分碳水化合物。饮食条件是随机的，受试者每种饮食间隔7-14天。每次饮食结束后，进行功率自行车运动(峰值耗氧量的70%)至力竭。分别于运动前和运动后取股外侧肌活检。分别于安静时、运动30min后、运动结束时经股动脉和股静脉导管采集血样。腿部耐力为66。+-。4和79。+-。安慰剂和DHAP后2分钟(P<；0.01)。休息和疲劳时的肌肉糖原在不同的饮食中没有区别。静息状态下，DHAP组的全腿动静脉血糖差值(P<；0.05)大于安慰剂组(0.36±-)。0.05比0.19。+-0.07 mm)和运动30分钟后(1.06±-)。0.14比0.65+-0.10 mm)，但在力竭时差异不大。在休息和运动期间，两种饮食的血浆游离脂肪酸、甘油和β-羟丁酸相似。运动过程中葡萄糖氧化总量估计为165。+-。17和203。+-。安慰剂和DHAP后分别为15g(P<；0.05)。结论：在高碳水化合物饮食的基础上，饲喂青心酮7天可通过增加肌肉对葡萄糖的摄取来提高腿部运动耐力。
The effects of dietary supplementation of dihydroxyacetone and pyruvate (DHAP) on metabolic responses and endurance capacity during leg exercise were determined in eight untrained males (20-30 yr). During the 7 days before exercise, a high-carbohydrate diet was consumed (70% carbohydrate, 18% protein, 12% fat; 35 kcal/kg body weight). One hundred grams of either Polycose (placebo) or dihydroxyacetone and pyruvate (treatment, 3:1) were substituted for a portion of carbohydrate. Dietary conditions were randomized, and subjects consumed each diet separated by 7-14 days. After each diet, cycle ergometer exercise (70% of peak oxygen consumption) was performed to exhaustion. Biopsy of the vastus lateralis muscle was obtained before and after exercise. Blood samples were drawn through radial artery and femoral vein catheters at rest, after 30 min of exercise, and at exercise termination. Leg endurance was 66 .+-. 4 and 79 .+-. 2 min after placebo and DHAP, respectively (P < 0.01). Muscle glycogen at rest and exhaustion did not differ between diets. Whole leg arteriovenous glucose difference was greater (P < 0.05) for DHAP than for placebo at rest (0.36 .+-. 0.05 vs. 0.19 .+-. 0.07 mM) and after 30 min of exercise (1.06 .+-. 0.14 vs. 0.65 .+-. 0.10 mM) but did not differ at exhaustion. Plasma free fatty acids, glycerol, and .beta.-hydroxybutyrate were similar during rest and exercise for both diets. Estimated total glucose oxidation during exercise was 165 .+-. 17 and 203 .+-. 15 g after placebo and DHAP, respectively (P < 0.05). It is concluded that feeding of DHAP for 7 days in conjunction with a high carbohydrate diet enhances leg exercise endurance capacity by increasing glucose extraction by muscle.