HEAT-PRODUCTION OF MAMMALIAN-CELLS AT DIFFERENT CELL-CYCLE PHASES
HEAT-PRODUCTION OF MAMMALIAN-CELLS AT DIFFERENT CELL-CYCLE PHASES
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DOI：
10.1016/0306-4565(82)90026-2
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
VANWIJK, R
中科院分区：
文献类型：
--
作者：
LOESBERG, C;VANMILTENBURG, JC;VANWIJK, R
用微量热法测定了H35大鼠肝癌细胞和C1300小鼠神经母细胞瘤细胞在细胞周期不同阶段的产热。将G1期细胞和S期细胞的H35单层培养物胰蛋白酶化，在悬浮培养物中再孵育，并立即用于微量热测量。G_1期细胞与S期细胞在单层培养的H_35细胞悬液中的放热有显著差异。G1细胞的产热在实验过程中是相对连续的，与S期细胞相比，S期细胞在初始最大值后产热减少。通过有丝分裂摇脱同步化并悬浮培养的神经母细胞瘤细胞在其细胞周期中进展。它们在有丝分裂前不久和有丝分裂期间表现出最大的产热。
Heat production of Reuber H35 rat hepatoma cells and murine C1300 neuroblastoma cells at different stages of the cell cycle were measured microcalorimetrically. Reuber H35 monolayer cultures of G1-phase cells and cells in S-phase were trypsinized, reincubated in suspension culture and immediately used for microcalorimetric measurements. There was a remarkable difference in the heat evolution of H35-cells in suspension derived from a monolayer culture of G1-phase cells and that of cells in S-phase of the cell cycle. Heat production of G1-cells was relatively continuous during the experiment, in contrast to S-phase cells that showed a decrease in heat production after an initial maximum. Neuroblastoma cells synchronized by mitotic shake-off and cultured in suspension progressed through their cell cycle. They showed maximal heat production shortly before and during mitosis.