NUCLEOTIDE-SEQUENCE OF A MUTANT EUKARYOTIC GENE - YEAST TYROSINE-INSERTING OCHRE SUPPRESSOR SUP4-O
NUCLEOTIDE-SEQUENCE OF A MUTANT EUKARYOTIC GENE - YEAST TYROSINE-INSERTING OCHRE SUPPRESSOR SUP4-O
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DOI：
10.1073/pnas.74.12.5453
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发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
HALL, BD
中科院分区：
文献类型：
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作者：
GOODMAN, HM;OLSON, MV;HALL, BD
与酵母tRNATyr杂交的酵母DNA的8个内切核酸酶EcoRI片段之一被鉴定为具有遗传上定义的无义抑制基因座SUP4。通过分析SUP4表型的遗传决定子和EcoRI片段的电泳变体之间的减数分裂连锁来实现这种鉴定。然后从抑制赭石的酵母菌株中克隆SUP4基因，并通过DNA测序进行分析。还对野生型SUP4基因和另外2个遗传上未鉴定的tRNAtyr基因进行了测序。赭石抑制基因的序列与野生型基因的序列的不同之处在于G → C → D。T. cntdot.编码tRNATyr反密码子的摆动位置碱基的碱基对中的颠换。所有4个基因在反密码子三联体的3“侧都含有一个14个碱基对的片段，这在成熟的tRNATyr中是不存在的。虽然代表3个非连锁染色体基因座的4个基因都编码相同的成熟tRNA序列，但在成熟tRNATyr序列的5“末端之前的区域中的3个基因座之间实际上没有可观察到的序列同源性。
One of the 8 endonuclease EcoRI fragments of yeast DNA that hybridize to yeast tRNATyr was identified with the genetically defined nonsense-suppressor locus SUP4. This identification was achieved by analyzing the meiotic linkage between the genetic determinant for the SUP4 phenotype and that for an electrophoretic variant of the EcoRI fragment. The SUP4 gene was then cloned from an ochre-suppressing yeast strain and analyzed by DNA sequencing. A wild-type SUP4 gene and 2 other genetically unidentified tRNAtyr genes were also sequenced. The sequence of the ochre suppressor differs from that of the wild-type genes by virtue of a G.cntdot.C .fwdarw. T.cntdot.A transversion in the base pair that codes for the wobble position base of the tRNATyr anticodon. All 4 genes contain, immediately to the 3'' side of the anticodon triplet, a 14 base pair tract that is not present in mature tRNATyr. Although the 4 genes, which represent 3 unlinked chromosomal loci, all encode the same mature tRNA sequence, there is virtually no observable sequence homology between the 3 loci in the region preceding the 5'' end of the mature tRNATyr sequences.