Muscovy duck retinoic acid-induced gene I (MdRIG-I) functions in innate immunity against H9N2 avian influenza viruses (AIV) infections
Muscovy duck retinoic acid-induced gene I (MdRIG-I) functions in innate immunity against H9N2 avian influenza viruses (AIV) infections
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番鸭视黄酸诱导基因 I (MdRIG-I) 在针对 H9N2 禽流感病毒 (AIV) 感染的先天免疫中发挥作用
DOI：
10.1016/j.vetimm.2014.12.009
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发表时间：
2015-02-15
影响因子：
1.8
通讯作者：
Sun, Jianhe
中科院分区：
文献类型：
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作者：
Cheng, Yuqiang;Huang, Qingqing;Sun, Jianhe
视黄酸诱导基因I（RIG-I）是一种感知病原体相关分子模式（PAMPs）的胞质模式识别受体。番鸭（Cairinamoschata）是一种不同于其它鸭类的大型鸭，对某些病原微生物较易感。本研究对番鸭RIG-I基因（MdRIG-I）进行了鉴定。定量RT-PCR结果显示，MdRIG-I mRNA在不同组织中均有表达，以黏膜组织表达最多。用编码MdRIG-I或CARD结构域的DNA构建体转染的RIG-I无效DF-1细胞可激活IRF-3和NF-κ B以上调IFN-β启动子的活性。哺乳动物细胞中RIG-I下游信号通路的组分包括IRF-3、NF-κ B、IFN-β和IFN刺激的基因Mx-1、PKR和MDA 5在CARD 5过表达的DF-1细胞中显著上调。结果表明，RIG-I在H9 N2亚型禽流感病毒（AIV）攻击鸭后，其在脑、脾、肺和腔上囊中的表达均显著上调，其6个内部基因与H7N9和H10 N8亚型禽流感病毒密切相关。在体外，用MdRIG-I质粒转染的DF-1细胞可以显著响应H9 N2 AIV，通过增强IFN-β启动子活性和降低病毒滴度来证明。这些结果表明，MdRIG-I是RLR基因家族的新成员，参与抗病毒天然免疫的早期阶段。(C)2014爱思唯尔有限公司版权所有。
Retinoic acid inducible gene I (RIG-I) is a cytosolic pattern recognition receptor that senses pathogen-associated molecular patterns (PAMPs). Muscovy duck (Cairina moschata) is a large duck different from other species of ducks, and is more susceptible to some microbial pathogens. In this study, the Muscovy duck RIG-I gene (MdRIG-I) was identified. Quantitative RT-PCR showed that MdRIG-I mRNA was widely expressed in different tissues, especially in those with mucosa. RIG-I null DF-1 cells transfected with DNA constructs encoding MdRIG-I or CARDs domain can activate IRF-3 and NF-kappa B to up-regulated activity of IFN-beta promoter. The components of the signaling pathway downstream of RIG-I in mammalian cells including IRF-3, NF-kappa B, IFN-beta and the IFN-stimulated genes Mx-1, PKR and MDA5 were significantly up-regulated in CARD5-overexpressing-DF-1 cells. Implicating RIG-I in the antiviral response to an infection in vivo, we found that RIG-I expression in brain, spleen, lung and bursa were up-regulated in ducks challenged with H9N2 avian influenza virus (AIV), whose six internal genes were closely related to the H7N9 and H10N8 AIV. In vitro, DF-1 cells transfected with MdRIG-I plasmid can respond significantly to H9N2 AIV, evident through enhancement of IFN-beta promoter activity and decreased virus titer. Altogether, these results indicated that MdRIG-I is a novel member of RLR gene family, engaging in the early stage of antiviral innate immunity. (C) 2014 Elsevier B.V. All rights reserved.