EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice
EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice
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DOI：
10.1038/ncomms2396
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发表时间：
2013-02-01
影响因子：
16.6
通讯作者：
Zhang, Dabing
中科院分区：
文献类型：
--
作者：
Niu, Ningning;Liang, Wanqi;Zhang, Dabing
细胞程序性死亡对多细胞生物的发育是必不可少的，但植物细胞程序性死亡的途径及其调控仍然是一个谜。在这里，我们报告的ETERNAL TAPETUM 1，一个基本的螺旋-环-螺旋转录因子在陆地植物中保守，积极调节水稻花药绒毡层细胞的程序性细胞死亡。EAT 1表现出延迟的绒毡层细胞死亡和败育的花粉形成。ETERNAL TAPETUM 1直接调节OsAP 25和OsAP 37的表达，OsAP 25和OsAP 37编码天冬氨酸蛋白酶，其诱导酵母和植物中的程序性细胞死亡。表达和遗传分析表明，ETERNAL TAPETUM 1在绒毡层变性迟缓（TAPETUMDEGENERATION RETARDATION）的下游起作用，这是绒毡层程序性细胞死亡的另一个正调节因子，并且ETERNAL TAPETUM 1还可以与TAPETUMDEGENERATION RETARDATION蛋白相互作用。这项研究表明，ETERNAL TAPETUM 1促进天冬氨酸蛋白酶触发植物程序性细胞死亡，并揭示了一个动态的调节级联在水稻雄性生殖发育。
Programmed cell death is essential for the development of multicellular organisms, yet pathways of plant programmed cell death and its regulation remain elusive. Here we report that ETERNAL TAPETUM 1, a basic helix-loop-helix transcription factor conserved in land plants, positively regulates programmed cell death in tapetal cells in rice anthers. eat1 exhibits delayed tapetal cell death and aborted pollen formation. ETERNAL TAPETUM 1 directly regulates the expression of OsAP25 and OsAP37, which encode aspartic proteases that induce programmed cell death in both yeast and plants. Expression and genetic analyses revealed that ETERNAL TAPETUM 1 acts downstream of TAPETUM DEGENERATION RETARDATION, another positive regulator of tapetal programmed cell death, and that ETERNAL TAPETUM 1 can also interact with the TAPETUM DEGENERATION RETARDATION protein. This study demonstrates that ETERNAL TAPETUM 1 promotes aspartic proteases triggering plant programmed cell death, and reveals a dynamic regulatory cascade in male reproductive development in rice.