Lipid alterations in LLC-PK1 cells exposed to mercuric chloride.
Lipid alterations in LLC-PK1 cells exposed to mercuric chloride.
复制标题
暴露于氯化汞的 LLC-PK1 细胞中的脂质变化。
DOI：
10.1038/ki.1986.31
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发表时间：
1986
影响因子：
19.6
通讯作者：
Venkatachalam,MA
中科院分区：
文献类型：
--
作者：
Troyer,DA;Kreisberg,JI;Venkatachalam,MA
暴露于氯化汞的llc - pk1细胞的脂质改变。我们研究了hgcl2对LLC-PK1（猪肾）上皮细胞脂质的影响。我们的研究结果表明，用hgcl2处理细胞会导致未酯化脂肪酸（特别是花生四烯酸）和溶血磷脂的快速积累。通过气相色谱法和脂质磷法测定，未酯化花生四烯酸增加27倍，溶血磷脂酰乙醇胺（LPE）增加17倍，同时磷脂酰乙醇胺的质量下降26%。用14c硬脂酸、3H花生四烯酸或14c乙酸对脂质进行标记的细胞进行hgcl2处理后，也观察到类似的变化，但放射性标记技术也发现溶血磷脂酰胆碱（LPC）中标记含量增加，磷脂酰胆碱相应减少。电镜显示，这些改变伴随着质膜上的气泡形成和不可逆损伤。通过向细胞中添加脂肪酸，研究了未酯化脂肪酸在损伤发病机制中的可能作用。向细胞中添加不饱和脂肪酸（油酸、亚油酸或花生四烯酸）会导致细胞膜起泡和丧失活力。同样，向细胞中添加LPC或LPE也会导致细胞死亡；然而，没有导致质膜起泡。
Lipid alterations in LLC-PK1cells exposed to mercuric chloride. We have studied the effects of HgCl2on the lipids of LLC-PK1(pig kidney) epithelial cells. Our results show that treatment of cells with HgCl2caused a rapid accumulation of unesterified fatty acids (particularly arachidonic acid) and lysophospholipids. A 27-fold increase in unesterified arachidonic acid and a 17-fold increase in lysophosphatidylethanolamine (LPE) was accompanied by a 26% decline in the mass of phosphatidylethanolamine as determined by gas chromatography and lipid phosphorus assay. Similar changes were seen following HgCl2treatment of cells whose lipids were labelled with14C stearic acid,3H arachidonic acid, or14C acetate, but the radiolabelling techniques also identified an increased content of label in lysophosphatidylcholine (LPC) and a corresponding decrease in phosphatidylcholine. These alterations were accompanied by the formation of blebs on the plasma membrane and irreversible injury as indicated by electron microscopy. The possible role of unesterified fatty acids in the pathogenesis of injury was studied by adding fatty acids to the cells. The addition of unsaturated fatty acids (oleic, linoleic, or arachidonic acids) to the cells caused plasma membrane blebbing and loss of viability. Similarly, the addition of LPC or LPE to the cells resulted in cell death; however, plasma membrane blebbing did not result.