A Novel Botulinum Neurotoxin, Previously Reported as Serotype H, Has a Hybrid-Like Structure With Regions of Similarity to the Structures of Serotypes A and F and Is Neutralized With Serotype A Antitoxin
A Novel Botulinum Neurotoxin, Previously Reported as Serotype H, Has a Hybrid-Like Structure With Regions of Similarity to the Structures of Serotypes A and F and Is Neutralized With Serotype A Antitoxin
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DOI：
10.1093/infdis/jiv327
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发表时间：
2016-02-01
影响因子：
6.4
通讯作者：
Johnson, Eric A.
中科院分区：
文献类型：
--
作者：
Maslanka, Susan E.;Luquez, Carolina;Johnson, Eric A.
肉毒中毒是一种由肉毒毒素作用于神经细胞而引起的具有潜在致命性的麻痹性疾病。已知BoNT有7种血清型（A-G）和多达40种遗传变异。最近有报道称，肉毒杆菌IBCA10-7060菌株可产生血清型B型BoNT （BoNT/B）和一种新型BoNT （BoNT/ H）。将BoNT/H的BoNT基因（BoNT）序列与已知的BoNT序列进行比较。遗传分析表明，BoNT/H具有与BoNT/A1和BoNT/F5结构相似的杂交样结构。这种新型BoNT通过小鼠中和试验和基于神经元细胞的试验进行血清学表征。这种杂交样BoNT的毒性作用被现有的血清A型抗毒素完全消除，包括那些包含在人类肉毒中毒治疗主要的多价治疗抗毒素产品中的抗毒素。
Botulism is a potentially fatal paralytic disease caused by the action of botulinum neurotoxin (BoNT) on nerve cells. There are 7 known serotypes (A-G) of BoNT and up to 40 genetic variants. Clostridium botulinum strain IBCA10-7060 was recently reported to produce BoNT serotype B (BoNT/B) and a novel BoNT, designated as BoNT/H. The BoNT gene (bont) sequence of BoNT/H was compared to known bont sequences. Genetic analysis suggested that BoNT/H has a hybrid-like structure containing regions of similarity to the structures of BoNT/A1 and BoNT/F5. This novel BoNT was serologically characterized by the mouse neutralization assay and a neuronal cell-based assay. The toxic effects of this hybrid-like BoNT were completely eliminated by existing serotype A antitoxins, including those contained in multivalent therapeutic antitoxin products that are the mainstay of human botulism treatment.