Determining the affinity of hormone-receptor interaction.
Determining the affinity of hormone-receptor interaction.
复制标题
确定激素-受体相互作用的亲和力。
DOI：
10.1007/978-1-60327-378-7_1
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Angelova,Krassimira
中科院分区：
文献类型：
--
作者：
Puett,David;Angelova,Krassimira
激素与其同源受体结合的表征是分子和细胞内分泌学中许多研究的基石，因为该事件代表特异性细胞应答的开始，通常来自高度调节的细胞外信使。激素-受体相互作用的前提是遵循质量作用定律，该定律描述了可逆的二级反应，即激素加受体，得到非共价缔合的激素-受体复合物。从这一基本原理出发，许多有用的实验参数可供感兴趣的研究者使用。本章重点是发展激素-受体相互作用的实验和数学基础，重点是促性腺激素，绒毛膜促性腺激素（或促黄体激素），结合促黄体激素受体，G蛋白偶联受体家族的成员。然而，本文中开发的一般概念和方法对于大多数交互系统是有效的。
Characterization of the binding of a hormone to its cognate receptor is a cornerstone of many studies in molecular and cellular endocrinology since this event represents the beginning of a specific cellular response, generally from a highly regulated extracellular messenger. The premise of hormone−receptor interaction follows from the law of mass action describing a reversible second-order reaction, hormone plus receptor, to give a non-covalently associated hormone-receptor complex. From this basic principle, a host of useful experimental parameters are available to the interested investigator. This chapter is focused on development of the experimental and mathematical underpinning of hormone−receptor interaction, with emphasis on a gonadotropin, chorionic gonadotropin (or luteinizing hormone), binding to the luteinizing hormone receptor, a member of the G protein-coupled receptor family. The general concepts and approaches developed herein are, however, valid to most interacting systems.