Genotypic characterization of an epithelial cell line for the study of parasite-epithelial interactions
Genotypic characterization of an epithelial cell line for the study of parasite-epithelial interactions
复制标题
DOI：
10.1645/ge-1395.1
复制
发表时间：
2008-04-01
影响因子：
1.3
通讯作者：
Lin, Yi-Chan
中科院分区：
文献类型：
--
作者：
Buret, Andre;Lin, Yi-Chan
本研究报告中讨论的结果报告了最初从人类患者获得的肠上皮细胞系的广泛基因型特征。尽管该品系表现出核型异常，具有76条模态染色体，但其免疫学、生化和生理表型使其成为研究肠上皮过程（包括肠寄生期间所涉及的过程）的非常合适的模型。聚合酶链反应（PCR），同工酶分析和PCR基因产物测序最终显示，SCBN上皮细胞表达犬基因型。这些观察结果适用于直接从实验室获得的早期细胞储备之一，其中细胞系首次分离。由于当时该实验室未使用犬细胞，并且考虑到正常犬模式染色体数为78，因此无法通过简单的实验室污染来解释SCBN细胞的犬基因型。尽管如此，SCBN的各种特性使其成为上皮生物学以及寄生虫-上皮相互作用研究的有用工具。其新发现的基因型特征对使用该细胞系的研究人员具有重要意义。
The findings discussed in the present research note report the extensive genotypic characterization of an intestinal epithelial cell line originally obtained from a human patient. Although the line exhibits karyotypic anomalies, with 76 modal chromosomes, its immunological, biochemical, and physiological phenotype make it a well-suited model to study intestinal epithelial processes, including those involved during intestinal parasitism. Polymerase chain reaction (PCR), isoenzyme analysis, and PCR gene product sequencing ultimately revealed that SCBN epithelial cells express a canine genotype. The observations held true for one of the early cell stocks obtained directly from the laboratory where the cell line was first isolated. Since no canine cells were used in that laboratory at that time, and in view of the normal canine modal chromosomal number of 78, the canine genotype of SCBN cells cannot be explained through simple laboratory contamination. The various characteristics of SCBN nonetheless make it a useful tool for research in epithelial biology, as well as in parasite-epithelial interactions. Its newly discovered genotypic characteristics are of significant relevance to researchers using this cell line.