Effect of miR-122 and its target gene cationic amino acid transporter 1 on colorectal liver metastasis
Effect of miR-122 and its target gene cationic amino acid transporter 1 on colorectal liver metastasis
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DOI：
10.1111/cas.12122
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发表时间：
2013-05-01
期刊：
影响因子：
5.7
通讯作者：
Konno, Hiroyuki
中科院分区：
文献类型：
--
作者：
Iino, Ichirota;Kikuchi, Hirotoshi;Konno, Hiroyuki
控制肝转移是结直肠癌（CRC）治疗中的一个重要问题。MicroRNA已被证明参与许多癌症的发展，但对其在结直肠肝转移过程中的作用知之甚少。我们比较了原发性结直肠肿瘤和肝转移瘤之间的miRNA表达，以确定参与转移过程的miRNA。使用激光捕获显微切割从福尔马林固定的石蜡包埋的原发性CRC样本及其相应的转移性肝肿瘤中分离癌细胞，并使用TaqMan miRNA阵列分析miRNA表达。与原发性肿瘤相比，肝转移中最丰富的miRNA是miR-122。免疫组化分析显示，miR-122的阴性靶基因阳离子氨基酸转运蛋白1（CAT 1）在肝转移瘤中的表达水平低于原发性肿瘤（P
Control of liver metastasis is an important issue in the treatment of colorectal cancer (CRC). MicroRNAs have been shown to be involved in the development of many cancers, but little is known about their role in the process of colorectal liver metastasis. We compared miRNA expression between primary colorectal tumors and liver metastasis to identify those involved in the process of metastasis. Cancer cells were isolated from formalin-fixed paraffin-embedded primary CRC samples and their corresponding metastatic liver tumors in six patients using laser capture microdissection, and miRNA expression was analyzed using TaqMan miRNA arrays. The most abundant miRNA in liver metastasis compared with primary tumors was miR-122. Immunohistochemical analysis revealed that the expression levels of cationic amino acid transporter 1 (CAT1), a negative target gene of miR-122, were lower in liver metastases than primary tumors (P