Ribosomal protein S27-like in colorectal cancer: a candidate for predicting prognoses.
Ribosomal protein S27-like in colorectal cancer: a candidate for predicting prognoses.
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DOI：
10.1371/journal.pone.0067043
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Chien CC
中科院分区：
文献类型：
--
作者：
Huang CJ;Yang SH;Lee CL;Cheng YC;Tai SY;Chien CC
结直肠癌（CRC）的发生和发展涉及多种遗传变化的复杂过程。肿瘤抑制因子p53能够决定CRC细胞的命运。然而，p53诱导的调节剂，核糖体蛋白S27样（RPS 27 L），在CRC中的作用是未知的。在此，研究了RPS 27 L在CRC患者粪便和结肠组织中的差异表达，以探索其与患者生存的可能相关性，并研究其临床结果的细胞机制。80例中期CRC患者（42例II期和38例III期）根据其粪便RPS 27 L mRNA水平分为两组。使用Kaplan-Meier方法估计各组的生存概率。进一步用免疫组化方法检测了不同疾病的III期患者结肠组织中的RPS 27 L蛋白。操纵LoVo细胞中的RPS 27 L表达以检查体外可能的细胞应答。粪便或组织中RPS 27 L表达升高与预后较好相关。在体外，表达RPS 27 L的LoVo细胞停止了DNA合成和凋亡活性，同时其DNA修复分子的表达上调。RPS 27 L升高可能会通过增强结肠细胞的DNA修复能力来改善某些CRC患者的预后，并且可以在粪便中确定。通过整合临床，分子和细胞数据，我们的研究表明，粪便RPS 27 L可能是预测癌症和指导个性化治疗策略的有用指标，特别是在中期CRC患者中。
The development and progression of colorectal cancer (CRC) involve a complex process of multiple genetic changes. Tumor suppressor p53 is capable of determining the fate of CRC cells. However, the role of a p53-inducible modulator, ribosomal protein S27-like (RPS27L), in CRC is unknown. Here, the differential expression of RPS27L was examined in the feces and colonic tissues of CRC patients, to explore its possible correlation with patient survival and to investigate the cellular mechanisms underlying their clinical outcomes. Eighty intermediate-stage CRC patients (42 at stage II and 38 at stage III) were divided into two groups according to their fecal RPS27L mRNA levels. The survival probabilities of the groups were estimated using the Kaplan–Meier method. The RPS27L protein in the colonic tissues of stage III patients with different prognoses was further examined immunohistochemically. RPS27L expression in LoVo cells was manipulated to examine the possible cellular responses in vitro. Elevated RPS27L expression, in either feces or tissues, was related to a better prognosis. In vitro, RPS27L-expressing LoVo cells ceased DNA synthesis and apoptotic activity while the expression of their DNA repair molecules was upregulated. Elevated RPS27L may improve the prognoses of certain CRC patients by enhancing the DNA repair capacity of their colonic cells, and can be determined in feces. By integrating clinical, molecular, and cellular data, our study demonstrates that fecal RPS27L may be a useful index for predicting prognoses and guiding personalized therapeutic strategies, especially in patients with intermediate-stage CRC.
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影响因子：
50.5
作者：
Farina-Sarasqueta, A.;van Lijnschoten, G.;van den Brule, A. J. C.
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