Control of visually guided behavior by distinct populations of spinal projection neurons
Control of visually guided behavior by distinct populations of spinal projection neurons
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DOI：
10.1038/nn2048
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发表时间：
2008-03-01
影响因子：
25
通讯作者：
Engert, Florian
中科院分区：
文献类型：
--
作者：
Orger, Michael B.;Kampff, Adam R.;Engert, Florian
运动控制领域的一个基本问题是，不同的动作是如何通过脊髓投射神经元的活动来表示的。我们使用了一种新的行为分析来识别专门驱动斑马鱼基本运动模式的视觉刺激。这些刺激诱发了投射到脊髓的神经元中的神经活动的一致模式，我们可以使用体内双光子钙成像在整个人群中进行映射。我们发现，驱动不同行为的刺激激活了投射神经元的不同子集，在某些情况下，这些神经元仅由几个细胞组成。这与更复杂行为的分布式激活形成对比。此外，有针对性的细胞与诱发轮相关的神经元消融取消了相应的行为反应。这种描述的斑马鱼运动系统的功能组织提供了一个框架，用于确定脊椎动物行为的完整电路。
A basic question in the field of motor control is how different actions are represented by activity in spinal projection neurons. We used a new behavioral assay to identify visual stimuli that specifically drive basic motor patterns in zebrafish. These stimuli evoked consistent patterns of neural activity in the neurons projecting to the spinal cord, which we could map throughout the entire population using in vivo two-photon calcium imaging. We found that stimuli that drive distinct behaviors activated distinct subsets of projection neurons, consisting, in some cases, of just a few cells. This stands in contrast to the distributed activation seen for more complex behaviors. Furthermore, targeted cell by cell ablations of the neurons associated with evoked turns abolished the corresponding behavioral response. This description of the functional organization of the zebrafish motor system provides a framework for identifying the complete circuit underlying a vertebrate behavior.