A brain tumor molecular imaging strategy using a new triple-modality MRI-photoacoustic-Raman nanoparticle
A brain tumor molecular imaging strategy using a new triple-modality MRI-photoacoustic-Raman nanoparticle
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DOI：
10.1038/nm.2721
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发表时间：
2012-05-01
期刊：
影响因子：
82.9
通讯作者：
Gambhir, Sanjiv S.
中科院分区：
文献类型：
--
作者：
Kircher, Moritz F.;de la Zerda, Adam;Gambhir, Sanjiv S.
描绘脑肿瘤边缘的困难是脑肿瘤患者获得更好治疗结果的主要障碍。目前的成像方法往往受到灵敏度、特异性和空间分辨率不足的限制。在这里，我们展示了一种独特的三模态磁共振成像-光声成像-拉曼成像纳米颗粒（这里称为MPR纳米颗粒）可以准确地帮助描绘活小鼠术前和术中脑肿瘤的边缘。在体外和活体小鼠中，所有三种方法都能检测到mpr，灵敏度至少为皮摩尔。将MPR静脉注射到患有胶质母细胞瘤的小鼠体内，导致肿瘤积累和保留MPR，而周围的健康组织中没有MPR积累，从而允许使用所有三种方式通过完整的颅骨进行非侵入性肿瘤描绘。拉曼成像可指导术中肿瘤切除，组织学相关性证实拉曼成像能准确描绘脑肿瘤边缘。这种新的三重模式纳米颗粒方法有望实现更准确的脑肿瘤成像和切除。
The difficulty in delineating brain tumor margins is a major obstacle in the path toward better outcomes for patients with brain tumors. Current imaging methods are often limited by inadequate sensitivity, specificity and spatial resolution. Here we show that a unique triple-modality magnetic resonance imaging-photoacoustic imaging-Raman imaging nanoparticle (termed here MPR nanoparticle) can accurately help delineate the margins of brain tumors in living mice both preoperatively and intraoperatively. The MPRs were detected by all three modalities with at least a picomolar sensitivity both in vitro and in living mice. Intravenous injection of MPRs into glioblastoma-bearing mice led to MPR accumulation and retention by the tumors, with no MPR accumulation in the surrounding healthy tissue, allowing for a noninvasive tumor delineation using all three modalities through the intact skull. Raman imaging allowed for guidance of intraoperative tumor resection, and a histological correlation validated that Raman imaging was accurately delineating the brain tumor margins. This new triple-modality-nanoparticle approach has promise for enabling more accurate brain tumor imaging and resection.