Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates.
Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates.
复制标题
通过 PCR 筛选 trbB 和 traG 样序列，用于检测细菌土壤分离物中的接合质粒。
DOI：
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发表时间：
2001
影响因子：
1.5
通讯作者：
B. Dreiseikelmann
中科院分区：
文献类型：
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作者：
C. Disqué;A. Battermann;M. Strätz;B. Dreiseikelmann
不同接合质粒的转移区域在遗传组织和某些基因产物的氨基酸序列中显示出显著的相似性，特别是来自traG或trbB家族的蛋白质。这些相似性在核苷酸序列水平上也是明显的。根据保守的DNA区域，我们设计了简并PCR引物对，以检测从农业土壤中分离的细菌克隆的集合中的特异性tra区域。大多数潜在的转移熟练的土著细菌分离株能够动员的非接合IncQ质粒RSF1010到受体菌株的衍生物。在引物的帮助下，应该可以评估接合质粒进行水平基因转移的遗传潜力。
The transfer regions of different conjugative plasmids show significant similarities in the genetic organization and in the amino acid sequence of some gene products, especially of proteins from the traG or trbB family. These similarities are also evident on the level of the nucleotide sequences. On the basis of conserved DNA regions we designed degenerate PCR primer pairs to detect specifically tra regions within a collection of bacterial clones isolated from an agricultural soil. Most of the potential transfer-proficient indigenous bacterial isolates were able to mobilize a derivative of the nonconjugative IncQ plasmid RSF1010 into recipient strains. With the help of the primers it should be possible to evaluate the genetic potential for horizontal gene transfer carried out by conjugative plasmids.