HIGH RESOLUTION AUTORADIOGRAPHY WITH DRY MOUNTED, FREEZE-DRIED FROZEN SECTIONS COMPARATIVE STUDY OF SIX METHODS USING TWO DIFFUSIBLE COMPOUNDS H-ESTRADIOL AND H-MESOBILIRUBINOGEN
HIGH RESOLUTION AUTORADIOGRAPHY WITH DRY MOUNTED, FREEZE-DRIED FROZEN SECTIONS COMPARATIVE STUDY OF SIX METHODS USING TWO DIFFUSIBLE COMPOUNDS H-ESTRADIOL AND H-MESOBILIRUBINOGEN
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使用两种扩散化合物 H-雌二醇和 H-中胆红素原进行干式冷冻切片的高分辨率放射自显影六种方法的比较研究
DOI：
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发表时间：
1966
影响因子：
3.2
通讯作者：
L. J. Roth
中科院分区：
文献类型：
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作者：
W. Stumpf;L. J. Roth
本文用六种放射自显影方法研究了两种可溶性化合物，~ 3 H-雌二醇在肝脏和子宫中的定位及~ 3 H-中胆红素原在肝脏中的定位：（Ⅰ）冷冻干燥干封于乳胶包被载玻片上的冰冻切片，（Ⅱ）解冻于乳胶包被载玻片上的冰冻切片，（Ⅲ）解冻于玻璃载玻片上的冰冻切片，（Ⅳ）用液体乳剂浸渍的冰冻切片，（Ⅳ）用液体乳剂浸渍的冰冻切片。（IV）冷冻干燥的组织，蒸汽固定，环氧包埋，切片，浸入液体乳液中;（V）冷冻干燥的组织，石蜡包埋，切片，脱蜡，浸入液体乳液中;和（VI）福尔马林固定的组织，石蜡包埋，切片，脱蜡，浸入液体乳液中。方法II-VI显示不同程度的扩散，并在某些情况下，从组织的活动损失，当与冷冻干燥的冷冻切片干安装获得的结果相比。已发现，将0.75 μ和1 μ厚的切片在-60 ° C以下切割并干燥后，通过干封片制备的放射自显影图具有上级优势。
Localization of two soluble compounds, 3H-estradiol in liver and uterus and 3H-mesobilirubinogen in liver, has been studied by six autoradiographic methods: (I) Frozen sections, freeze-dried, dry mounted on emulsion-coated slides; (II) Frozen sections thawed on emulsion-coated slides; (III) Frozen sections thawed on glass slides and dipped in liquid emulsion; (IV) Freeze-dried tissue, vapor-fixed, epoxy-embedded, sectioned, dipped in liquid emulsion; (V) Freeze-dried tissue, paraffin-embedded, sectioned, deparaffinized, dipped in liquid emulsion; and (VI) Formalin-fixed tissue, paraffin-embedded, sectioned, deparaffinized and dipped in liquid emulsion. Methods II-VI show varying degrees of diffusion, and in some cases loss of activity from tissue, when compared with results obtained by dry mounting of freeze-dried frozen sections. Autoradiograms prepared by dry mounting of 0.75-, and 1-µ thick sections, cut and dried below –60°C, have been found superior.