The generalized transducing Salmonella bacteriophage ES18:: Complete genome sequence and DNA packaging strategy
The generalized transducing Salmonella bacteriophage ES18:: Complete genome sequence and DNA packaging strategy
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DOI：
10.1128/jb.187.3.1091-1104.2005
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发表时间：
2005-02-01
影响因子：
3.2
通讯作者：
Hendrix, RW
中科院分区：
文献类型：
--
作者：
Casjens, SR;Gilcrease, EB;Hendrix, RW
广义转导双链DNA噬菌体ES 18具有二十面体的头部和长的非收缩性尾部，并且它感染粗糙和光滑的沙门氏菌菌株。我们在这里报告了完整的46，900 bp基因组核苷酸序列，并提供了序列分析。它的79个基因和它们的组织清楚地表明，ES 18是类噬菌体组的成员;然而，它包含一组新的基因，可以编程病毒体头部的组装。其大部分整合切除、免疫、Nin区和裂解基因与短尾沙门氏菌噬菌体P22的基因几乎相同，而其它早期基因与大肠杆菌Escherichia coli λ和HK 97、S.肠道噬菌体ST 64 T或福氏志贺氏菌前噬菌体。一些ES 18晚期基因是新的，而另一些则与N1 HK 97、lambda或N15最密切相关。因此，ES 18的基因组与其他类人猿的基因组是嵌合的，这对所有的组成员都是典型的。病毒体DNA的分析表明，它是环状排列的，约10%的末端冗余，DNA包装系列的起始发生在大约1 kbp的区域，而不是在基因组上的精确位置。这支持了一种模型，其中ES 18末端酶可以在识别和切割注定要包装的DNA之间沿着DNA移动沿着相当大的距离。大的末端酶亚基的生物信息学分析表明，噬菌体编码的末端酶的不同功能类别通常可以从它们的氨基酸序列预测。
The generalized transducing double-stranded DNA bacteriophage ES18 has an icosahedral head and a long noncontractile tail, and it infects both rough and smooth Salmonella enterica strains. We report here the complete 46,900-bp genome nucleotide sequence and provide an analysis of the sequence. Its 79 genes and their organization clearly show that ES18 is a member of the lambda-like (lambdoid) phage group; however, it contains a novel set of genes that program assembly of the virion head. Most of its integration-excision, immunity, Nin region, and lysis genes are nearly identical to those of the short-tailed Salmonella phage P22, while other early genes are nearly identical to Escherichia coli phages lambda and HK97, S. enterica phage ST64T, or a Shigella flexneri prophage. Some of the ES18 late genes are novel, while others are most closely related to phages HK97, lambda, or N15. Thus, the ES18 genome is mosaically related to other lambdoid phages, as is typical for all group members. Analysis of virion DNA showed that it is circularly permuted and about 10% terminally redundant and that initiation of DNA packaging series occurs across an approximately 1-kbp region rather than at a precise location on the genome. This supports a model in which ES18 terminase can move substantial distances along the DNA between recognition and cleavage of DNA destined to be packaged. Bioinformatic analysis of large terminase subunits shows that the different functional classes of phage-encoded terminases can usually be predicted from their amino acid sequence.