STRUCTURAL-PROPERTIES, FUNCTIONAL-STATES AND PHYSIOLOGICAL ROLES OF HYDROGENASE IN PHOTOSYNTHETIC BACTERIA
STRUCTURAL-PROPERTIES, FUNCTIONAL-STATES AND PHYSIOLOGICAL ROLES OF HYDROGENASE IN PHOTOSYNTHETIC BACTERIA
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DOI：
10.1111/j.1574-6968.1990.tb04945.x
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发表时间：
1990-12-01
影响因子：
2.1
通讯作者：
BAGYINKA, C
中科院分区：
文献类型：
--
作者：
KOVACS, KL;BAGYINKA, C
Thiocapsa roseopersicina的膜结合氢化酶由两个亚基组成，每个分子含有两个Fe-S中心和一个Ni。该酶具有耐热性和抗蛋白降解性，在SDA-PAGE条件下仍保持活性，用质子诱导X射线荧光分析（PIXE）确定了金属原子在亚基上的位置。一个修正的氢化酶模型，符合新的dates.The在光合作用的酶的方向和它的能力，以产生膜电位建议，氢化酶可以发挥重要作用，在这些细菌的能量。提出了固氮、光合电子传递和氢化酶之间可能的联系。
The membrane-bound hydrogenase from Thiocapsa roseopersicina is composed of two subunits and contains two Fe-S centres and one Ni per molecule. The enzyme resists heat and proteolytic degradation, its activity is retained under SDA-PAGE conditions.The location of metal atoms on the subunits has been determined by proton-induced X-ray emission (PIXE). A revised hydrogenase model which concurs with the new date is suggested.The orientation of the enzyme in the photosynthetic membrane and its ability to generate membrane potential suggest that hydrogenase can play a significant role in the energetics of these bacteria. A possible link between nitrogen fixation, photosynthetic electron transport and hydrogenase is proposed.