Immunological comparison of lipoxygenase isozymes-1 and -2 with soybean seedling lipoxygenases.
Immunological comparison of lipoxygenase isozymes-1 and -2 with soybean seedling lipoxygenases.
复制标题
脂氧合酶同工酶-1和-2与大豆幼苗脂氧合酶的免疫学比较。
DOI：
10.1016/0003-9861(85)90190-0
复制
发表时间：
1985
影响因子：
3.9
通讯作者：
Siedow,JN
中科院分区：
文献类型：
--
作者：
Peterman,TK;Siedow,JN
制备了大豆种子脂氧合酶-2的亲和纯化多克隆抗体，并用于表征脂氧合酶同工酶1和2与大豆幼苗根、下胚轴、叶和子叶中脂氧合酶的免疫相关性。所有测试的大豆脂氧合酶与抗脂氧合酶-2交叉反应。种子衍生的脂氧合酶的交叉反应性证明了双扩散试验中的身份线的形成，免疫印迹的阳性结果，和酶活性的抗体沉淀。水平的抗脂氧合酶-2，抑制脂氧合酶-2的活性，对脂氧合酶-1的活性没有影响。根，下胚轴，和叶脂氧合酶没有形成沉淀线在双扩散试验，但抗脂氧合酶-2抑制和沉淀脂氧合酶活性从这些来源以及交叉反应的免疫印迹。所有检查的交叉反应性脂氧合酶被发现具有相同的表观分子量。在大豆幼苗根、下胚轴、叶和子叶中发现的脂氧合酶活性与蛋白质相关，这些蛋白质都与种子来源的酶免疫相关。
An affinity-purified polyclonal antibody against soybean seed lipoxygenase-2 was prepared and used to characterize the immunological relatedness of lipoxygenase isozymes 1 and 2 and lipoxygenases from soybean seedling roots, hypocotyls, leaves, and cotyledons. All soybean lipoxygenases tested cross-reacted with the anti-lipoxygenase-2. Cross-reactivity of seed-derived lipoxygenases was evidenced by formation of a line of identity in double-diffusion tests, by positive results on an immunoblot, and by antibody precipitation of enzyme activity. Levels of anti-lipoxygenase-2, which inhibited lipoxygenase-2 activity, had no effect on lipoxygenase-1 activity. Root, hypocotyl, and leaf lipoxygenases did not form precipitation lines in double-diffusion tests but the anti-lipoxygenase-2 did inhibit and precipitate lipoxygenase activity from these sources as well as cross-react on immunoblots. All the cross-reactive lipoxygenases examined were found to have the same apparent molecular weight. Lipoxygenase activity found in soybean seedling roots, hypocotyls, leaves, and cotyledons is associated with proteins which are all immunologically related to the seed-derived enzymes.