Genetic Diversity of Avian Infectious Bronchitis Virus Isolates in Korea Between 2003 and 2006
Genetic Diversity of Avian Infectious Bronchitis Virus Isolates in Korea Between 2003 and 2006
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DOI：
10.1637/8117-092707-resnote.1
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发表时间：
2008-06-01
期刊：
影响因子：
1.4
通讯作者：
Choi, Kang-Seuk
中科院分区：
文献类型：
--
作者：
Lee, Eun-Kyoung;Jeon, Woo-Jin;Choi, Kang-Seuk
2003-2006年间，从韩国商品鸡群中分离到33株鸡传染性支气管炎病毒(IBV)野毒株，通过对IBV S1基因高变区的核苷酸序列分析，对IBV进行了系统发育分析。我们的系统发育分析表明，最近分离的IBV野毒株至少形成了三种不同的系统发育类型，包括K-I、K-II和K-III。K-I型IBV由Kr-EJ/95株进化而来的13株IBV本土分离株组成，并分为K-Ia型和K-Ib型。K-II型IBV分离株(n=19)与中国和日本的肾性IBV变异株关系密切。本研究首次鉴定的K-III型分离株(Kr/D064/05)与引起腺胃炎的中国株肠道IBV变异株密切相关。序列比较表明，IBV型间~gt；氨基酸差异达27.5%。简要讨论了IBV野毒分离株的分子流行病学特征。
Thirty-three field isolates of avian infectious bronchitis virus (IBV) were recovered from commercial chicken flocks in Korea between 2003 and 2006 and were characterized phylogenetically by nucleotide sequence analysis of the IBV S1 gene hyper-variable region. Our phylogenetic analysis revealed that recent field isolates of IBV formed at least three distinct phylogenetic types, including K-I, K-II, and K-III. K-I type IBV consisted of indigenous, 13 IBV isolates which evolved from the Kr-EJ/95 strain and then separated into the lineages of type K-Ia and type K-Ib. K-II type IBV isolates (n = 19) were closely related to nephropathogenic IBV variants from China and Japan. The K-III type isolate (Kr/D064/05), first identified by this study, was closely related to enteric IBV variants from the Chinese strains that cause proventriculitis. Sequence comparisons showed amino acid differences of >27.5% between IBV types. The molecular epidemiologic characteristics of IBV field isolates are briefly discussed.