Cilia play a role in breaking left-right symmetry of the sea urchin embryo.
Cilia play a role in breaking left-right symmetry of the sea urchin embryo.
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纤毛在打破海胆胚胎的左右对称性方面发挥着重要作用。
DOI：
10.1111/gtc.12362
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发表时间：
2016
期刊：
影响因子：
2.1
通讯作者：
Sakamoto N
中科院分区：
文献类型：
--
作者：
Takemoto A;Miyamoto T;Simono F;Kurogi N;Kurabayashi MS;Awazu A;Suzuki KT;Yamamoto T;Sakamoto N
两侧对称动物的左右不对称是在早期发育过程中形成的。在小鼠、青蛙和鱼类中，纤毛左右组织者在建立双侧不对称性中起着重要作用，纤毛运动产生的细胞外液的逆向流动导致左侧的Nodal活动。然而，H+/K+-ATP酶活性也参与了多种动物左右不对称的决定，并且被认为是后口动物的祖先机制。在海胆中，基于H+/K+-ATP酶活性的左右不对称性的确定已经澄清，但纤毛运动是否涉及胚胎的左右不对称性仍有待发现。在这里，我们证明纤毛运动参与建立左右不对称的海胆胚胎的证据。此外，我们表明，在胚胎发生的早期阶段，在小微粒上产生的初始纤毛可能参与了这一过程。这些结果表明，纤毛介导的左右不对称性的决定机制可能是在后口动物的基部获得的。
Left–right asymmetry of bilaterian animals is established during early development. In mice, frogs and fishes, the ciliated left–right organizer plays an essential role in establishing bilateral asymmetry, and leftward flow of extracellular fluid generated by ciliary motion results in Nodal activity on the left side. However, H+/K+‐ATPase activity is also involved in the determination of left–right asymmetry in a variety of animals, and it has been thought to be an ancestral mechanism in deuterostomes. In sea urchin, the determination of the left–right asymmetry based on H+/K+‐ATPase activity was already clarified, but it remains to be uncovered whether ciliary motion is involved in the left–right asymmetry of the embryo. Here, we show evidence that ciliary motion is involved in the establishment of left–right asymmetry of sea urchin embryo. Furthermore, we show that the initial cilia generated on small micromeres during the early stage of embryogenesis may be involved in this process. These results suggest that the cilia‐mediated mechanism for the determination of left–right asymmetry may be acquired at the base of the deuterostomes.