Biological and molecular characterization of a canine hemangiosarcoma-derived cell line
Biological and molecular characterization of a canine hemangiosarcoma-derived cell line
复制标题
DOI：
10.1016/j.rvsc.2005.09.005
复制
发表时间：
2006-08-01
影响因子：
2.4
通讯作者：
MacEwen, EG
中科院分区：
文献类型：
--
作者：
Thamm, DH;Dickerson, EB;MacEwen, EG
犬血管肉瘤（HSA）是一种毁灭性的疾病。新疗法的研究受到犬HSA衍生细胞系有限可用性的限制。我们报告了犬HSA衍生细胞系DEN-HSA的发展，该细胞系概括了血管生成内皮的特征。DEN-HSA细胞来源于狗肾脏中产生的自发HSA。DEN-HSA显示内皮组织发生的表面分子，包括因子VIII相关抗原，ICAM-1和003整合素。在体外，DEN-HSA在Matrigel上形成微血管管状结构，并响应于多种血管生成生长因子而增殖。细胞表达bFGF及其受体、VEGF及其受体等特异性mRNA和蛋白质。DEN-HSA条件培养基在小鼠角膜囊袋试验中诱发了显着的血管生成反应，表明该细胞系分泌的物质具有强效的促血管生成活性。这项研究证实了DEN-HSA细胞系作为内皮细胞的起源，表明存在血管生成生长因子自分泌环，并提供了利用DEN-HSA细胞研究调节内皮细胞增殖的新疗法的潜力。(c)2005年由Elsevier Ltd.出版
Canine hemangiosarcoma (HSA) is a devastating disease. Investigation of novel therapies has been limited by the limited availability of canine HSA-derived cell lines. We report the development of a canine HSA-derived cell line, DEN-HSA, which recapitulates features of angiogenic endothelium. DEN-HSA cells were derived from a spontaneous HSA arising in the kidney of a dog. DEN-HSA displayed surface molecules distinctive of endothelial histogenesis, including factor VIII-related antigen, ICAM-1 and 003 integrin. In vitro, DEN-HSA formed microvascular tube-like structures on Matrigel (R), and proliferated in response to a variety of angiogenic growth factors. The cells expressed mRNA and protein specific for bFGF and its receptors, and VEGF and its receptors, among others. DEN-HSA conditioned medium evoked a marked angiogenic response in a murine corneal pocket assay, indicating potent proangiogenic activity of substances secreted by this cell line. This research confirms the DEN-HSA cell line as endothelial in origin, suggests the presence of angiogenic growth factor autocrine loops, and offers the potential to utilize DEN-HSA cells for the study of novel therapies that modulate endothelial proliferation. (c) 2005 Published by Elsevier Ltd.