Urine nicotine metabolite concentrations in relation to plasma cotinine during low-level nicotine exposure
Urine nicotine metabolite concentrations in relation to plasma cotinine during low-level nicotine exposure
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DOI：
10.1093/ntr/ntp092
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发表时间：
2009-08-01
影响因子：
4.7
通讯作者：
Jacob, Peyton, III
中科院分区：
文献类型：
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作者：
Benowitz, Neal L.;Dains, Katherine M.;Jacob, Peyton, III
简介：血浆或唾液可替宁浓度被广泛用作二手烟（SHS）暴露的生物标志物，并与SHS相关疾病的风险相关。尿液中可替宁和其他尼古丁代谢物的浓度远高于血浆或唾液，使化学分析更容易。此外，在一些SHS暴露研究中，尿液通常更便于收集。尚未确定尿液中尼古丁代谢物的最佳使用（单独或组合，校正或不校正尿肌酸酐浓度），以估计低水平尼古丁暴露的血浆可替宁浓度。方法：我们给36个不吸烟的人注射了100，200，或400 mg氘标记尼古丁（模拟暴露于SHS），持续5天，然后在24小时内以不同的间隔测量血浆和尿可替宁和代谢物。血浆可替宁浓度为1 ng/ml时，平均相当于每日摄入100 mg尼古丁。尿液中的可替宁浓度平均为血浆浓度的4至5倍。尿可替宁肌酐浓度的校正改善了尿液和血浆可替宁之间的相关性。测量尿液中的多种可替宁代谢产物并没有改善与血浆可替宁的相关性，与使用尿可替宁alternating.Discussion：尿可替宁校正肌酐浓度的测量似乎是血浆可替宁的最佳预测因子。
Introduction: Plasma or saliva cotinine concentrations are used widely as biomarkers of secondhand smoke (SHS) exposure and have been associated with the risk of SHS-related disease. Concentrations of cotinine and other nicotine metabolites are considerably higher in urine than in plasma or saliva, making chemical analysis easier. In addition, urine is often more convenient to collect in some SHS exposure studies. The optimal use of nicotine metabolites in urine, singly or in combination, with or without correction for urine creatinine concentration, to estimate plasma cotinine concentration with low-level nicotine exposure has not been determined.Methods: We dosed 36 nonsmokers with 100, 200, or 400 m g deuterium-labeled nicotine (simulating exposure to SHS) by mouth daily for 5 days and then measured plasma and urine cotinine and metabolites at various intervals over 24 hr.Results: A plasma cotinine concentration of 1 ng/ml corresponds on average to a daily intake of 100 m g nicotine. Cotinine concentrations in urine averaged four to five times those in plasma. Correction of urine cotinine for creatinine concentration improved the correlation between urine and plasma cotinine. Measuring multiple cotinine metabolites in urine did not improve the correlation with plasma cotinine, compared with the use of urine cotinine alone.Discussion: Measurement of urine cotinine corrected for creatinine concentration appears to be the best predictor of plasma cotinine.