Immobilization of Arg-Gly-Asp (RGD) sequence in a thermosensitive hydrogel for cell delivery using pheochromocytoma cells (PC12)
Immobilization of Arg-Gly-Asp (RGD) sequence in a thermosensitive hydrogel for cell delivery using pheochromocytoma cells (PC12)
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DOI：
10.1016/s1389-1723(04)70221-2
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发表时间：
2004-06-01
影响因子：
2.8
通讯作者：
Yun, K
中科院分区：
文献类型：
--
作者：
Park, KH;Yun, K
采用N-异丙基丙烯酰胺(进料摩尔分数为98%)-丙烯酸共聚物[p(NiPAAm-co-AAc)]和黏附分子Arg-Gly-Asp(RGD)包埋嗜铬细胞瘤细胞(PC12)。在28d的培养周期中，RGD偶联凝胶中的PC12细胞保持了较高的存活率，并以恒定的速率产生多巴胺，而p(NiPAAm-co-AAC)中的PC12细胞存活率较低，分泌的多巴胺较少。在RGD-偶联凝胶中培养的PC12细胞将构成一个潜在的应用于神经再生的三维细胞系统。
A copolymer of N-isopropylacrylamide (98 mole% in the feed) and acrylic acid poly(N-isopropylacrylamide-co-acrylic acid) [p(NiPAAm-co-AAc)], and the adhesion molecule, an Arg-Gly-Asp (RGD)-incorporated hydrogel, were used to entrap pheochromocytoma cells (PC12). In a 28-d culture period, the PC12 cells in the RGD-conjugated gel maintained higher viability and produced dopamine at constant rates, while there was lower cell viability and less dopamine secretion by PC12 cells in p(NiPAAm-co-AAc). PC12 cells cultured in the RGD-conjugated gel would constitute a potentially useful three-dimensional cell system for application in nerve regeneration.