Genome-wide DNA methylation in 1-year-old infants of mothers with major depressive disorder.
Genome-wide DNA methylation in 1-year-old infants of mothers with major depressive disorder.
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DOI：
10.1017/s0954579416000912
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发表时间：
2016-11
影响因子：
3.3
通讯作者：
Toth SL
中科院分区：
文献类型：
--
作者：
Cicchetti D;Hetzel S;Rogosch FA;Handley ED;Toth SL
对 114 名婴儿样本（M 年龄 = 13.2 个月；SD = 1.08）进行了全基因组甲基化研究，这些婴儿是患有（n=73）和不患有（n=41）重度抑郁症（MDD）的低收入城市妇女。使用带有 GenomeStudio 甲基化模块和 Illumina Custom 模型的 Illumina HumanMmethylation450 BeadChip 阵列进行差异甲基化分析。使用 5.0 × 10−7 p 值，发现 2119 个基因座在抑郁母亲和非抑郁母亲的婴儿之间存在显着差异。与非抑郁母亲的婴儿相比，抑郁母亲的婴儿在低甲基化位点（0-29%）有更高的甲基化。在高甲基化水平（70-100%）下，抑郁母亲的婴儿主要是低甲基化。抑郁症母亲的婴儿与非抑郁症母亲的婴儿的甲基化平均差异为 5.23%。还使用 GeneGo MetaCore 软件进行了疾病生物标志物分析。结果表明生物标志物网络中存在显着的癌症相关差异，例如前列腺肿瘤、卵巢和乳腺肿瘤以及结肠肿瘤。过程网络分析的结果表明，抑郁症和非抑郁症母亲的婴儿之间与神经元发育和中枢神经系统功能以及心脏发育相关的过程网络存在显着差异。这些发现表明，在发育早期，患有重度抑郁症的母亲所生的婴儿与健康母亲所生的婴儿相比，表现出表观遗传差异，这表明存在日后不良健康结果的风险。
A genome-wide methylation study was conducted among a sample of 114 infants (M age = 13.2 mo.; SD = 1.08) of low-income urban women with (n=73) and without (n=41) Major Depressive Disorder (MDD). The Illumina HumanMethylation450 BeadChip array with the GenomeStudio Methylation Module and Illumina Custom model were used to conduct differential methylation analyses. Using the 5.0 × 10−7 p-value, 2119 loci were found to be significantly different between infants of depressed and non-depressed mothers. Infants of depressed mothers had greater methylation at low methylation sites (0–29%) compared to infants of non-depressed mothers. At high levels of methylation (70–100%) the infants of depressed mothers were predominantly hypomethylated. The mean difference in methylation between the infants of depressed and infants of non-depressed mothers was 5.23%. Disease by biomarker analyses were also conducted using GeneGo MetaCore Software. Results indicated significant cancer-related differences in biomarker networks such as prostatic neoplasms, ovarian and breast neoplasms, and colonic neoplasms. Results of a process networks analysis indicated significant differences in process networks associated with neuronal development and central nervous system functioning, as well as cardiac development between infants of depressed and non-depressed mothers. These findings indicate that early in development, infants of mothers with MDD evince epigenetic differences relative to infants of well mothers that suggest risk for later adverse health outcomes.