Wireless Instantaneous Neurotransmitter Concentration System: electrochemical monitoring of serotonin using fast-scan cyclic voltammetry - a proof-of-principle study Laboratory investigation
Wireless Instantaneous Neurotransmitter Concentration System: electrochemical monitoring of serotonin using fast-scan cyclic voltammetry - a proof-of-principle study Laboratory investigation
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DOI：
10.3171/2010.3.jns091627
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发表时间：
2010-09-01
影响因子：
4.1
通讯作者：
Lee, Kendall H.
中科院分区：
文献类型：
--
作者：
Griessenauer, Christoph J.;Chang, Su-Youne;Lee, Kendall H.
Object.作者先前报告了用于测量多巴胺的无线瞬时神经递质浓度系统（WINCS）的开发，并建议该技术可用于评价脑深部电刺激对神经递质系统的神经调节作用。WINCS支持碳纤维微电极（CFM）上的快速扫描循环伏安法（FSCV），用于实时、空间分辨的神经递质测量。用于建立WINCS多巴胺测量的FSCV参数不适合于5-羟色胺，这是一种与抑郁症有关的神经递质，因为它们导致CFM污染和灵敏度丧失。在这里，作者将先前描述的N形波形以高扫描速率应用于WINCS以建立无线血清素监测。FSCV针对5-羟色胺的检测进行了优化，由N形波形组成，以1000 V/秒的速率从+0.2 V到+1.0 V，然后到-0.1 V，再回到+0.2 V的静息电位线性扫描。原理验证试验包括流动注射分析和电诱发大鼠脑片中缝背核5-羟色胺释放。流动池注射分析表明，相对于用多巴胺的FSCV参数观察到的情况，以1000 V/秒的扫描速率施加的N波形显著减少了CFM的血清素污染。在脑切片中，WINCS可靠地检测到由局部高频刺激引起的中缝背核中的亚秒级5-羟色胺释放。作者发现WINCS支持在CFM处通过FSCV进行高保真无线5-羟色胺监测。在未来，对大型动物模型和人类血清素的测量可能有助于建立深部脑刺激治疗精神疾病的机制。(DOI：10.3171/2010.3.JNS091627）
Object. The authors previously reported the development of the Wireless Instantaneous Neurotransmitter Concentration System (WINCS) for measuring dopamine and suggested that this technology may be useful for evaluating deep brain stimulation related neuromodulatory effects on neurotransmitter systems. The WINCS supports fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM) for real-time, spatially resolved neurotransmitter measurements. The FSCV parameters used to establish WINCS dopamine measurements are not suitable for serotonin, a neurotransmitter implicated in depression, because they lead to CFM fouling and a loss of sensitivity. Here, the authors incorporate into WINCS a previously described N-shaped waveform applied at a high scan rate to establish wireless serotonin monitoring.Methods. Optimized for the detection of serotonin, FSCV consisted of an N-shaped waveform scanned linearly from a resting potential of +0.2 to +1.0 V, then to -0.1 V and back to +0.2 V, at a rate of 1000 V/second. Proof-of-principle tests included flow injection analysis and electrically evoked serotonin release in the dorsal raphe nucleus of rat brain slices.Results. Flow cell injection analysis demonstrated that the N waveform, applied at a scan rate of 1000 V/second, significantly reduced serotonin fouling of the CFM, relative to that observed with FSCV parameters for dopamine. In brain slices, WINCS reliably detected subsecond serotonin release in the dorsal raphe nucleus evoked by local high-frequency stimulation.Conclusions. The authors found that WINCS supported high-fidelity wireless serotonin monitoring by FSCV at a CFM. In the future such measurements of serotonin in large animal models and in humans may help to establish the mechanism of deep brain stimulation for psychiatric disease. (DOI: 10.3171/2010.3.JNS091627)