Poly(dopamine) coating of 3D printed poly(lactic acid) scaffolds for bone tissue engineering
Poly(dopamine) coating of 3D printed poly(lactic acid) scaffolds for bone tissue engineering
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DOI：
10.1016/j.msec.2015.06.028
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发表时间：
2015-11-01
影响因子：
7.9
通讯作者：
Shie, Ming-You
中科院分区：
文献类型：
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作者：
Kao, Chia-Tze;Lin, Chi-Chang;Shie, Ming-You
3D打印是一种多功能技术，可以产生大量各种形状和尺寸的聚合物。本研究的目的是开发功能化3D打印聚乳酸（PLA）支架，并使用贻贝启发的表面涂层来调节人脂肪源性尾细胞（hADSCs）的细胞粘附、增殖和分化。我们制备了聚多巴胺包被的PIA 3D支架。用XPS表征了PDA/PLA的化学组成和表面性能。PDA/PLA通过几种方式调节hscs的反应。首先，相对于PLA， PDA/PLA培养的hscs的粘附、增殖和细胞周期明显增强。在成骨实验中，在PDA/PLA支架上培养的hscs的ALP活性和骨钙素显著高于在纯PIA支架上培养的hscs。此外，在PDA/PLA上培养的hscs显示与血管生成分化相关的ang-1和vWF蛋白上调。我们的研究结果表明，仿生涂层合成聚乳酸聚合物可以作为一种简单的技术，使合成支架表面具有活性，从而使它们能够指导hascs的特定反应。(C) 2015 Elsevier B.V.版权所有
3D printing is a versatile technique to generate large quantities of a wide variety of shapes and sizes of polymer. The aim of this study is to develop functionalized 3D printed poly(lactic acid) (PLA) scaffolds and use a mussel-inspired surface coating to regulate cell adhesion, proliferation and differentiation of human adipose-derived stern cells (hADSCs). We prepared PIA 3D scaffolds coated with polydopamine (PDA). The chemical composition and surface properties of PDA/PLA were characterized by XPS. PDA/PLA modulated hADSCs' responses in several ways. Firstly, adhesion and proliferation, and cell cycle of hADSCs cultured on PDA/PLA were significantly enhanced relative to those on PLA. In addition, the collagen I secreted from cells was increased and promoted cell attachment and cell cycle progression were depended on the PDA content In osteogenesis assay, the ALP activity and osteocalcin of hADSCs cultured on PDA/PLA were significantly higher than seen in those cultured on pure PIA scaffolds. Moreover, hADSCs cultured on PDA/PLA showed up-regulation of the ang-1 and vWF proteins associated with angiogenic differentiation. Our results demonstrate that the bio-inspired coating synthetic PLA polymer can be used as a simple technique to render the surfaces of synthetic scaffolds active, thus enabling them to direct the specific responses of hADSCs. (C) 2015 Elsevier B.V. All rights reserved.