Decreased expression of filaggrin in atopic skin
Decreased expression of filaggrin in atopic skin
复制标题
特应性皮肤中丝聚合蛋白的表达减少
DOI：
10.1007/bf02505232
复制
发表时间：
1996
影响因子：
3
通讯作者：
T. Tezuka
中科院分区：
文献类型：
--
作者：
T. Seguchi;Chang;S. Kusuda;Masae Takahashi;Kinue Aisu;T. Tezuka
采用免疫组织化学方法测量特应性皮炎 (AD) 患者皮损和非皮损皮肤中角质形成细胞终末分化过程中产生的表皮蛋白丝聚合蛋白、外皮蛋白、半胱氨酸蛋白酶抑制剂 A 和 Tad-H-1 抗原的量。此外，通过酶联免疫吸附测定（ELISA）技术测量了通过穿刺活检获得的AD患者上臂内表面（非病变皮肤）和正常对照的皮肤中丝聚蛋白的量。免疫组织化学研究表明，病变皮肤中所有四种蛋白质均减少。相比之下，AD 患者的非病变皮肤中只有聚丝蛋白减少。 ELISA结果显示，AD患者上臂内表面皮肤丝聚蛋白含量为2.48±0.45 μg/7 mm2（n=8），是正常对照者（7.7±0.55 μg/7 mm2；n=4）的32%。特应性皮肤中丝聚蛋白产生的减少可能是特应性皮肤容易变得干燥的原因之一，因为丝聚蛋白被认为是角质层中润肤因子的前体蛋白。尽管丝聚合蛋白和外皮蛋白基因位于同一基因 1q21 的非常有限的部分，但 AD 患者中只有丝聚合蛋白的表达受到抑制，这表明丝聚蛋白基因与外皮蛋白基因不共享调控元件。
The amounts of the epidermal proteins filaggrin, involucrin, cystatin A and Tad-H-1 antigen produced during the terminal differentiation of keratinocytes were immunohistochemically measured in lesional and nonlesional skin of atopic dermatitis (AD) patients. In addition, the amount of filaggrin in the skin of the inner surface of the upper arm of AD patients (nonlesional skin) and normal controls, obtained by punch biopsy, was measured by an enzyme-linked immunosorbent assay (ELISA) technique. The immunohistochemical study showed that all four proteins were decreased in lesional skin. By contrast, only filaggrin was decreased in nonlesional skin of AD patients. The ELISA showed that the amount of filaggrin in the skin of the inner surface of the upper arm was 2.48±0.45 μg/7 mm2 (n=8) in AD patients, which was 32% of that in the normal controls (7.7±0.55 μg/7 mm2;n=4). This decrease in filaggrin production in atopic skin may be one of the reasons why atopic skin can easily become dry, because filaggrin is thought to be the precursor protein of the emollient factors in the stratum corneum. The evidence that only the expression of filaggrin was suppressed in AD patients, though the genes of filaggrin and involucrin are localized to a very restricted portion of the same gene 1q21, indicates that the filaggrin gene does not share regulatory elements with the involucrin gene.
登录
查看更多内容
表皮蛋白酶对大鼠表皮高分子量富含组氨酸的蛋白质进行有限的蛋白水解。
DOI：
10.1111/1523-1747.ep12462067
发表时间：
1988
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Kashima,M;Fukuyama,K;Kikuchi,M;Epstein,WL
通讯作者：
Epstein,WL
影响因子：
6.5
作者：
SYBERT, VP;DALE, BA;HOLBROOK, KA
通讯作者：
HOLBROOK, KA
两种针对人表皮透明角质蛋白的单克隆抗体的表征：与聚丝蛋白和相关蛋白的反应性。
DOI：
10.1111/1523-1747.ep12466185
发表时间：
1987
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Dale,BA;Gown,AM;Fleckman,P;Kimball,JR;Resing,KA
通讯作者：
Resing,KA
人类外皮蛋白基因编码区缺少单个重复，导致 RFLP。
影响因子：
9.8
作者：
Simon,M;Phillips,M;Green,H;Stroh,H;Glatt,K;Burns,G;Latt,SA
通讯作者：
Latt,SA