From the Cover: The dynamics of genomic-length DNA molecules in 100-nm channels.
From the Cover: The dynamics of genomic-length DNA molecules in 100-nm channels.
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DOI：
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发表时间：
2004
影响因子：
11.1
通讯作者：
J. Tegenfeldt;C. Prinz;H. Cao;S. Chou;W. Reisner;R. Riehn;Yan Mei Wang;E. Cox;J. Sturm;
中科院分区：
文献类型：
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作者：
J. Tegenfeldt;C. Prinz;H. Cao;S. Chou;W. Reisner;R. Riehn;Yan Mei Wang;E. Cox;J. Sturm;
我们发现，基因组长度的DNA分子在纳米通道成像有一个扩展沿着的通道，与聚合物的轮廓长度线性缩放，在协议的缩放参数由德Gennes开发的自避免限制聚合物。这种基本关系使我们能够直接测量被限制在通道中的单个DNA分子的轮廓长度，并且对纳米通道中聚合物的动力学的统计分析使我们能够计算延伸平均值的SD。这种统计分析使我们能够在1分钟内测量具有130 nm SD的λ DNA多聚体的延伸。
We show that genomic-length DNA molecules imaged in nanochannels have an extension along the channel that scales linearly with the contour length of the polymer, in agreement with the scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly the contour length of single DNA molecules confined in the channels, and the statistical analysis of the dynamics of the polymer in the nanochannel allows us to compute the SD of the mean of the extension. This statistical analysis allows us to measure the extension of lambda DNA multimers with a 130-nm SD in 1 min.