Nanobdella aerobiophila gen. nov., sp. nov., a thermoacidophilic, obligate ectosymbiotic archaeon, and proposal of Nanobdellaceae fam. nov., Nanobdellales ord. nov. and Nanobdellia class. nov.
Nanobdella aerobiophila gen. nov., sp. nov., a thermoacidophilic, obligate ectosymbiotic archaeon, and proposal of Nanobdellaceae fam. nov., Nanobdellales ord. nov. and Nanobdellia class. nov.
复制标题
DOI：
10.1099/ijsem.0.005489
复制
发表时间：
2022-01-01
影响因子：
2.8
通讯作者：
Ohkuma, Moriya
中科院分区：
文献类型：
--
作者：
Kato, Shingo;Ogasawara, Ayaka;Ohkuma, Moriya
从日本的一个陆地温泉获得了一种新型嗜热嗜酸、专性共生古菌（命名为菌株 MJ1T）与其特定宿主古菌 Metallosphaera sedula 菌株 MJ1HA 的共培养物。菌株 MJ1T 在有氧条件下在共培养物中生长。菌株MJ1T的球状细胞直径为200-500 nm，并附着在共培养物中的MJ1HA细胞上。共培养中菌株MJ1T的生长温度和最适pH范围分别为60-75℃（最适65-70℃）和pH 1.0-4.0（最适pH 2.5）。从共培养物浓缩的MJ1T细胞中二烷基甘油四醚(GDGT)-3和GDGT-4的核心脂质高度丰富。 MJ1T 菌株的基因组较小 (0.67 Mbp)，缺乏用于生物合成必需生物分子（如核苷酸、脂质和 ATP）的基因。基因组DNA G+C含量为24.9mol%。菌株 MJ1T 的 16S rRNA 基因序列与栽培物种“Nanopusillus Acidilobi”菌株 N7A 的 16S rRNA 基因序列关系最密切（相似性为 85.8%）。基于系统发育和生理学特征，我们建议将其命名为 Nanobdella aerobiophila gen。十一月，SP。十一月以适应菌株 MJ1T (=JCM 33616T=DSM 111728T)。此外，我们建议将其命名为 Nanobdellaceae fam。 11 月，纳米菌目序。十一月，和 Nanobdellia 类。十一月以适应新的属。
A co- culture of a novel thermoacidophilic, obligate symbiotic archaeon, designated as strain MJ1T, with its specific host archaeon Metallosphaera sedula strain MJ1HA was obtained from a terrestrial hot spring in Japan. Strain MJ1T grew in the co- culture under aerobic conditions. Coccoid cells of strain MJ1T were 200-500 nm in diameter, and attached to the MJ1HA cells in the co- culture. The ranges and optima of the growth temperature and pH of strain MJ1T in the co- culture were 60-75 degrees C (optimum, 65-70 degrees C) and pH 1.0-4.0 (optimum, pH 2.5), respectively. Core lipids of dialkyl glycerol tetraethers (GDGT)-3 and GDGT- 4 were highly abundant in MJ1T cells concentrated from the co- culture. Strain MJ1T has a small genome (0.67 Mbp) lacking genes for biosynthesis of essential biomolecules, such as nucleotides, lipids and ATP. The genomic DNA G+C content was 24.9 mol%. The 16S rRNA gene sequence of strain MJ1T was most closely related to that of the cultivated species, 'Nanopusillus acidilobi' strain N7A (85.8 % similarity). Based on phylogenetic and physiological characteristics, we propose the name Nanobdella aerobiophila gen. nov., sp. nov. to accommodate the strain MJ1T (=JCM 33616T=DSM 111728T). In addition, we propose the names Nanobdellaceae fam. nov., Nanobdellales ord. nov., and Nanobdellia class. nov. to accommodate the novel genus.