Single-base resolution methylome analysis shows epigenetic changes in Arabidopsis seedlings exposed to microgravity spaceflight conditions on board the SJ-10 recoverable satellite
Single-base resolution methylome analysis shows epigenetic changes in Arabidopsis seedlings exposed to microgravity spaceflight conditions on board the SJ-10 recoverable satellite
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单碱基分辨率甲基化组分析显示，在SJ-10返回式卫星上暴露于微重力太空飞行条件下的拟南芥幼苗的表观遗传变化
DOI：
10.1038/s41526-018-0046-z
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发表时间：
2018-07-12
期刊：
影响因子：
5.1
通讯作者：
Cai, Weiming
中科院分区：
文献类型：
--
作者：
Xu, Peipei;Chen, Haiying;Cai, Weiming
DNA甲基化是一种非常重要的表观遗传修饰，参与许多生物学功能。尽管已经报道了许多关于不同植物物种DNA甲基化的研究，但很少有研究评估了暴露于微重力条件下植物的全球DNA甲基化模式。在本报告中，我们以单碱基分辨率绘制了中国返回式科学卫星SJ-10上与微重力条件相关的拟南芥基因组甲基化模式变化。有趣的是，我们发现了暴露于微重力环境下的拟南芥幼苗的表观遗传差异，在微重力条件下，CHG、CHH和CpG环境下，拟南芥基因组的甲基化水平较低。微重力刺激与许多基因的甲基化改变有关，包括DNA甲基化相关基因、激素信号相关基因、细胞壁修饰基因和转座因子（te）。相对不稳定的te DNA甲基化是诱导活跃转座子的原因。这些观察结果表明，te内的DNA去甲基化可能会影响转座子在微重力条件下的转录。综上所述，本研究结果有助于了解植物对微重力的适应机制，完善植物对空间的适应策略。
DNA methylation is a very important epigenetic modification that participates in many biological functions. Although many studies of DNA methylation have been reported in various plant species, few studies have assessed the global DNA methylation pattern in plants challenged by exposure to microgravity conditions. In this report, we mapped the Arabidopsis genome methylation pattern changes associated with microgravity conditions on board the Chinese recoverable scientific satellite SJ-10 at single-base resolution. Interestingly, we found epigenetic differences in Arabidopsis seedlings exposed to microgravity in that the Arabidopsis genome exhibits lower methylation levels in the CHG, CHH, and CpG contexts under microgravity conditions. Microgravity stimulation was related to altered methylation of a number of genes, including DNA methylation-associated genes, hormone signaling related genes, cell-wall modification genes and transposable elements (TEs). Relatively unstable DNA methylation of TEs was responsible for the induction of active transposons. These observations suggest that DNA demethylation within TEs may affect the transcription of transposons in response to microgravity conditions. In summary, the results of this investigation are beneficial for understanding the mechanism of plant adaptation to microgravity and improve strategies to allow plants to adapt to space.