Clostridium tyrobutyricum alleviates Staphylococcus aureus-induced endometritis in mice by inhibiting endometrial barrier disruption and inflammatory response
Clostridium tyrobutyricum alleviates Staphylococcus aureus-induced endometritis in mice by inhibiting endometrial barrier disruption and inflammatory response
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酪丁酸梭菌通过抑制子宫内膜屏障破坏和炎症反应减轻金黄色葡萄球菌引起的小鼠子宫内膜炎
DOI：
10.1039/c9fo00654k
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发表时间：
2019-10-01
期刊：
影响因子：
6.1
通讯作者：
Fu, Yunhe
中科院分区：
文献类型：
--
作者：
Hu, Xiaoyu;Guo, Jian;Fu, Yunhe
子宫内膜炎是一种由细菌感染引起的子宫炎症性疾病，影响人类和动物的健康。本研究旨在探讨酪丁酸梭菌（C. tyrobutyricum）等益生菌对金黄色葡萄球菌（S. aureus）诱发的子宫内膜炎的保护作用和分子机制。结果表明，金黄色葡萄球菌感染显着诱导子宫病理损伤，增加TNF-α和IL-1β等促炎细胞因子的产生，并减弱子宫组织紧密连接蛋白的表达。然而，酪丁酸梭菌预处理明显减轻了金黄色葡萄球菌引起的炎症反应并逆转了子宫紧密连接蛋白的变化。总之，数据表明酪丁酸梭菌还抑制金黄色葡萄球菌诱导的 TLR2/NF-kappa B 信号通路和 HDAC 的表达。此外，用活酪丁酸梭菌、来自酪丁酸梭菌的废培养物上清液(SCS)而不是非活性酪丁酸梭菌治疗小鼠，抑制了金黄色葡萄球菌诱导的炎症反应。通过进一步的研究，我们发现接受酪丁酸梭菌治疗的小鼠血液和子宫组织中的丁酸盐水平均显着升高。这些发现强调了酪丁酸梭菌通过增强子宫屏障完整性和抑制炎症反应对子宫内膜炎的保护作用。抗炎机制可能是通过调节 TLR2/NF-κB 和 HDAC 的表达来实现的，酪丁酸梭菌可能是治疗子宫内膜炎的潜在候选药物。
Endometritis is an inflammatory disease of the uterus caused by bacterial infection, and it affects both human and animal health. This study aims to investigate the protective effects and molecular mechanisms of probiotics such as Clostridium tyrobutyricum (C. tyrobutyricum) on Staphylococcus aureus (S. aureus)-induced endometritis. The results showed that S. aureus infection significantly induced the pathological damage of the uterus, increased the production of pro-inflammatory cytokines, such as TNF-alpha and IL-1 beta, and attenuated the expression of tight junction proteins of uterine tissues. However, C. tyrobutyricum pretreatment obviously reduced the inflammatory response and reversed the changes of tight junction proteins of the uterus induced by S. aureus. Together, the data showed that C. tyrobutyricum also inhibited the expression of the TLR2/NF-kappa B signaling pathway and HDAC induced by S. aureus. In addition, the treatment of mice with live C. tyrobutyricum, spent culture supernatants (SCS) from C. tyrobutyricum, rather than inactive C. tyrobutyricum, inhibited the inflammatory response induced by S. aureus. Through further research, we found that the levels of butyrate in both blood and uterine tissues of mice treated with C. tyrobutyricum were significantly increased. These findings underscore the protective effect of C. tyrobutyricum on endometritis by enhancing the uterus barrier integrity and inhibiting the inflammatory response. The anti-inflammatory mechanism may occur through the regulation of the expression of TLR2/NF-kappa B and HDAC, and C. tyrobutyricum can be a potentially therapeutic candidate for the treatment of endometritis.